PROTEIN CATABOLISM - 2 Decarboxylation—the-COOH group is...

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PROTEIN CATABOLISM Intact proteins cannot cross bacterial plasma membrane, so bacteria must produce extracellular  enzymes called proteases and peptidases that break down the proteins into amino acids, which  can enter the cell. Many of the amino acids are used in building bacterial proteins, but some may  also be broken down for energy. If this is the way amino acids are used, they are broken down to  some form that can enter the Kreb’s cycle. These reactions include:  1. Deamination—the amino group is removed, converted to an ammonium ion, and excreted. The  remaining organic acid (the part of the amino acid molecule that is left after the amino group is  removed) can enter the Kreb’s cycle.
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Unformatted text preview: 2. Decarboxylation—the ---COOH group is removed 3. Dehydrogenation—a hydrogen is removed Tests for the presence of enzymes that allow various amino acids to be broken down are used in identifying bacteria in the lab. WHY does everything used to produce energy wind up in the Kreb’s cycle??? BIOCHEMICAL TESTS Biochemical tests are frequently used in the lab to identify bacteria and other microbes. Morphology, gram-staining reaction, other special stains, etc. cannot come near identifying all existing types of bacteria, so biochemical tests are often required. These tests are really tests for the ability of the bacteria to make certain enzymes....
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