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Unformatted text preview: as this makes it sound! 1. The gene for the desired protein is obtained from a donor chromosome. 2. The donated gene is incorporated into a plasmid. 3. Plasmid-donor gene complex is carried into susceptible cell through the process of transformation. 4. Cells successfully receiving the plasmid-donor gene complex are isolated, identified, and cultured. These cells will have the ability to produce whatever protein the gene normally codes for. These cells can be grown in culture to form a clone, which contains identical cells all having the new gene. 5. Protein product is recovered and purified....
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This note was uploaded on 01/23/2012 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.
- Fall '09