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SPECIAL STAINS - SPECIALSTAINS 1. capsule., technique Steps...

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SPECIAL STAINS  1.  CAPSULAR   STAIN ----some types of bacteria are surrounded by a slimy outer covering called a  capsule. The capsule does not accept colored dyes, so the procedure involves a negative staining  technique.   Steps:               A drop of nigrosin or India ink is applied to one end of a clean slide. Bacteria are mixed into the drop The mixture is spread to form a thin film over the slide, which is allowed to  air dry. The slide is gently heat-fixed. Apply crystal violet for I minute and very gently  rinse. Blot dry and examine.   The capsules should appear as clear areas against a dark background.  Inside  each capsule, a purple bacterium should be seen.     2 ENDOSPORE   STAIN -----Endospores are special dormant forms of bacteria that are highly resistant  to adverse conditions such as heat, drying, toxic chemicals, lack of nutrients, etc.  Since only  a 
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