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Unformatted text preview: STAINING TECHNIQUES • Simple stains---one stain is applied to a heat-fixed smear and rinsed off. All microbes would take on the color of the dye. • Differential stains---stains will react differently with different types of bacteria GRAM STAIN This is the most important staining technique in microbiology. It was developed in 1884 by Dr. Christian Gram. It is a differential stain used to sort bacteria into 2 groups, gram-positive and gram-negative, which is the first big step in identification. The gram reaction may also aid in determining the proper treatment of the disease. Steps in the gram stain: • Crystal violet is applied to a heat-fixed smear for 30 seconds and gently rinsed off. This is the primary stain. • The smear is thoroughly covered with Gram’s iodine , which is left for 1 minute and then rinsed. The iodine acts as a mordant, fixing the crystal violet into the cell wall of gram-rinsed....
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This note was uploaded on 01/23/2012 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.
- Fall '09