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Unformatted text preview: Antibiotic Sensitivity Test -- Disk-plate (diffusion) method 1. Procedure a. Isolate and identify causative pathogen b. Grow pathogen in pure culture c. Seed surface agar plate - inoculate entire surface with pure culture d. Place paper antibiotic disks on surface of agar e. Incubate 24 hours, observe growth around disks 2. Principle: during incubation antibiotic diffuses into agar, if effective, inhibits growth 3. Observations: a. Zone of inhibition (no growth) - effective (positive) b. No zone of inhibition (growth) - not effective (negative) c. Resistant strains - isolated colonies in zone of inhibition 1...
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- Fall '09