706_S2006_Exam2 - Name: _ 7.06 Cell Biology EXAM #2 March...

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Name: ___________________________________ 1 7.06 Cell Biology EXAM #2 March 21, 2006 This is an OPEN BOOK exam, and you are allowed access to books, a calculator, and notes BUT NOT computers or any other types of electronic devices. Please write your answers in PEN (not pencil) to the questions in the space allotted. Please write only on the FRONT SIDE of each sheet, as we will Xerox all of the exams and thus only grade writing on the front. And be sure to put your NAME ON EACH PAGE in case they become separated! There are TEN pages including this cover sheet. Question 1. 20 pts __________ Question 2. 24 pts __________ Question 3. 26 pts __________ Question 4. 30 pts __________ TOTAL 100 pts __________
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___________________________________ 2 Question 1. (20 pts) You are studying the Wingless/Wnt pathway in mammalian cells. Your cells contain a reporter gene construct that consists of the open reading frame of GFP driven by the promoter for hedgehog. The type of cells you are using do indeed regulate expression from this reporter gene construct in response to extracellular Wnt as expected. (a, 6 pts) When would your reporter gene construct be expressed if the following mutant versions of Wnt pathway components were ectopically expressed in wild-type cells? YOUR CHOICES ARE: never, only + Wnt, only – Wnt, OR both + and – Wnt. (i) A version of beta-catenin in which all of its phosphorylation sites have been changed to alanine. (ii) A version of GSK3 that cannot interact with Dishevelled. (iii) A mutant form of Frizzled that does not localize to the membrane. (b, 6 pts) For each mutant ( i through iii ) above, when would your reporter gene construct be expressed if the same three mutant proteins were expressed in cells lacking the corresponding wild-type protein? (i) A version of beta-catenin in which all of its phosphorylation sites have been changed to alanine. (ii)
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This note was uploaded on 01/23/2012 for the course BIOLOGY lsm1301 taught by Professor Seow during the Spring '11 term at National University of Singapore.

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706_S2006_Exam2 - Name: _ 7.06 Cell Biology EXAM #2 March...

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