706_S2006_Exam3 - Name: _________________________________ 1...

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Unformatted text preview: Name: _________________________________ 1 7.06 Cell Biology EXAM #3 May 2, 2006 This is an OPEN BOOK exam, and you are allowed access to books, a calculator, and notes BUT NOT computers or any other types of electronic devices. Please write your answers in PEN (not pencil) to the questions in the space allotted. Please write only on the FRONT SIDE of each sheet, as we will Xerox all of the exams and thus only grade writing on the front. And be sure to put your NAME ON EACH PAGE in case they become separated! There are SEVEN pages including this cover sheet. Question 1. 24 pts __________ Question 2. 15 pts __________ Question 3. 27 pts __________ Question 4. 19 pts __________ Question 5. 15 pts __________ TOTAL 100 pts __________ Name: _________________________________ 2 Question 1. (24 pts.) For each of the following engineered proteins, predict which location in the cell they would be targeted to. Assume that each targeting sequence is at the site along the protein (N terminus, C terminus, internal) where it would normally be found. Also assume that the only targeting sequences possessed by each protein are those listed. Choose from the choices : the cytoplasm, the nucleus, the nuclear membrane, the peroxisome, the peroxisomal membrane, the outer mitochondrial membrane, the intermembrane space of the mitochondria, the inner mitochondrial membrane, the mitochondrial matrix, the lumen of the ER, or the membrane of the ER. (a, 4 pts) an NES and a mitochondrial matrix targeting sequence (b, 4 pts) a cleavable signal sequence and a stop transfer sequence (c, 4 pts) a mitochondrial matrix targeting sequence and a stop transfer sequence (d, 4 pts) a mitochondrial matrix targeting sequence and a sequence for phosphorylation of mannose residues on the 6 carbon atom...
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706_S2006_Exam3 - Name: _________________________________ 1...

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