706_S2006_PS8 - 7.06 Problem Set #8, 2006 1. You are...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
1 7.06 Problem Set #8, 2006 1. You are working in a cancer research lab studying tumorigenesis in mice. a) There are lots of genes that your lab might study! List all of the genes in the following pathways that we have learned about throughout the semester that are oncogenes, and all of the genes that are tumor suppressor genes. i) The Wingless pathway ii) The RTK/Ras pathway b) One of the assays your lab uses is the soft agar colony forming assay. What is the principle behind this assay? c) One of the genes studied in your lab is E2F, which is a transcripton factor that controls the G1 to S transition in mammals. i) How is E2F normally regulated in the cell? ii) How can E2F be inappropriately activated to lead to cancer? iii) How could you show experimentally that an excess of free E2F can lead to tumorigenesis? (Make sure to include controls in your experiment.) d) Another of the genes studied in your lab is Ras. Which of the following changes to wild-type Ras would make it oncogenic? -- if Ras cannot interact with Raf -- if Ras cannot hydrolyze GTP -- if Ras cannot interact with Sos -- if the farnesyl lipid anchor cannot be added to Ras
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 e) You have a mouse strain that is predisposed to developing skin cancer. Based on the literature in the cancer field, you come up with a list of 10 potential candidate genes that might be mutated in these mice. You are convinced that the mice of this strain must harbor one of these mutations. How would you determine which candidate gene from the list was actually mutated in this mouse? f) When you do the experiment listed above to determine which candidate gene is responsible for the predisposition to developing skin cancer, do you need to take tissue samples from the tumor itself, or can you take tissue from anywhere in the mouse? g) You also have a large colony of wild-type mice that you maintain in the lab. Once of these mice develops a disease that looks very similar to chronic myelogenous leukemia (CML). Knowing that a mutation in the c-abl gene often leads to CML in humans, you decide to test your tumorigenic mouse for mutations in c-abl . You design primers upstream and downstream of the c-abl open reading frame and try to perform PCR to amplify the c-abl gene from cancerous cells that you have harvested from your tumorigenic mouse. When you do PCR from a wild-type mouse on c-abl , the PCR reaction works just fine. You cannot get a c-abl PCR product from your tumorigenic mouse, however, no matter how much tweaking of reaction conditions you do. Explain why you can’t get a PCR product for c-abl in the tumorigenic mouse. h) Knowing what you know about the c-abl oncogene, how could you use PCR to test for the mutation in your tumorigenic mouse? 2.
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/23/2012 for the course BIOLOGY lsm1301 taught by Professor Seow during the Spring '11 term at National University of Singapore.

Page1 / 6

706_S2006_PS8 - 7.06 Problem Set #8, 2006 1. You are...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online