706_F06pset1 - 7.06 FALL 2006 Problem Set#1 1a In order to better understand the function of the yeast protein Yup1 you are interested in learning

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7.06 Fall 2006 PSet 1 1 7.06 FALL 2006 Problem Set #1 1a. In order to better understand the function of the yeast protein Yup1, you are interested in learning where Yup1 localizes inside the cell using immunofluorescence (IF). In order to save your PI money, you decide to make a monoclonal antibody to Yup1 yourself, therefore you inject purified Yup1 protein into a mouse. After you have isolated the B lymphocytes from the spleen, what do you do to generate an antibody? b. Congratulations, you have successfully made a specific antibody to Yup1. Once you have made a slide with fixed yeast cells, how can you now use that antibody to visualize the localization of Yup1; will you need to use anything besides your antibody? c. Based on the localization of Yup1 to the edge of the nucleus, you hypothesize that this protein might actually be residing in the nucleolus (a sub-compartment of the nucleus that houses the rDNA). How might you test that hypothesis and what reagents would you use to do so? d.
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This note was uploaded on 01/23/2012 for the course BIOLOGY lsm1301 taught by Professor Seow during the Spring '11 term at National University of Singapore.

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706_F06pset1 - 7.06 FALL 2006 Problem Set#1 1a In order to better understand the function of the yeast protein Yup1 you are interested in learning

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