706_F06pset2 - 7.06 FALL 2006 Problem Set #2 1. De s i gn...

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7.06 Fall 2006 PSet 2 1 of 2 7.06 FALL 2006 Problem Set #2 1. Design an experiment that would show that Raf lies downstream of Ras. What kind of results would you expect? 2. You wonder whether Ras and Raf interact directly (i.e. Do they bind to each other?). Design an experiment that could answer this question and explain what kind of results you would expect. 3. What would be the effect of the following mutations on the RAS signaling pathway activated by Epidermal Growth Factor (EGF)? a.) RAS protein can bind but not hydrolyze GTP. b.) SOS (GEF) protein with mutated proline rich region. c.) EGF Receptor (EGFR) with all Tyrosines mutated to Phenylaline. 4. You are working on a new growth hormone. You have cloned a receptor and found a novel kinase, FAB2, which seems to be activated by addition of the growth hormone. You think FAB2 may be involved in the MAP kinase signaling pathway. a.) How could you determine if the MAPK is the direct target protein for FAB2? b.) How could you identify the residues on MAPK phosphorylated by FAB2 activity? 5. As you know, yeast comes in two different mating types: a and alpha. Each secretes a different mating factor recognized by receptors on the cell surface which in turn results in activation of the MAP kinase pathway and transcription of genes that inhibit progression of the cell cycle and allow for diploid formation. You are interested in dissecting this pathway:
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This note was uploaded on 01/23/2012 for the course BIOLOGY lsm1301 taught by Professor Seow during the Spring '11 term at National University of Singapore.

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706_F06pset2 - 7.06 FALL 2006 Problem Set #2 1. De s i gn...

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