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Unformatted text preview: Problem Set #6 1. You are studying the effects of the TSG1 tumor suppressor gene in mice. You knockout one copy of the TSG1 gene in mice and you derive cells from these animals (They are now heterozygotes, +/-). As expected, the tsg1-knockout mutation is fully recessive at the cellular level. You examine tumor formation in mice and observe the following: % of mice w/ no tumors time WT tsg1 +/- A. If the tsg +/- mutation is recessive in cells, why do you observe an effect on tumor development in the animals? Next you add some suspected cancer-causing chemicals, PCBs, to their food. You watch for tumor formation in all mice and see the following: % o f m i c e w / n o t u m o r s time WT tsg1 +/- WT & PCB tsg1 +/- & PCB B. Explain why the tsg +/- animals developed tumors more rapidly than WT animals when ex- posed to PCBs. C. You breed two tsg +/- mice before they develop tumors. Six months later, both of the parent mice have developed tumors. You breed the parent mice again to see the effect of the parental tumors on have developed tumors....
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