2005_Exam4 - Name: _ 7.06 Cell Biology EXAM #4 May 18, 2005...

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Name: ________________________________ 7.06 Cell Biology EXAM #4 May 18, 2005 This is an open book exam, and you are allowed access to books, a calculator, and notes but not computers or any other types of electronic devices. Please write your answers in pen (not pencil) to the questions in the space allotted. Please write only on the FRONT SIDE of each sheet. And be sure to put your name on each page in case they become separated! Remember that we will Xerox all of the exams. Good luck! Question 1. 18 pts __________ Question 2. 17 pts __________ Question 3. 30 pts __________ Question 4. 17 pts __________ Question 5. 18 pts __________
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Name: ________________________________ 10 min 20 min 30 min 40 min 10 min 20 min 30 min 40 min Question 1. (18 pts) DIRECTIONS: For each of the situations depicted in parts a) , b) , and c) , Name one example of a mutational event in a specific gene that would cause the defect shown in the data, AND… Discuss how this specific mutation explains mechanistically why the cells used in the experiment have been transformed into cancerous cells. (a, 6 pts) You perform the following experiment on normal cells and cancerous cells from a specific colon cancer. You incubate the cells with a short pulse of radioactive amino acids, and then chase for a long period of time with non-radioactive amino acids. You wait for different amounts of time during the chase, and immunoprecipitate β - catenin. You run the IPs on an SDS-PAGE gel and expose the gel to autoradiography film. The film you see is as follows, with the bands shown being β -catenin: NORMAL COLON CELLS CANCEROUS COLON CELLS
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Name: ________________________________ (b, 6 pts) You perform a Southern blot on normal skin cells and cancerous skin cells. The radioactive probe you use is specific for the open reading frame of wild-type myc. You expose your gel to autoradiography film. The film you see is as follows, with the bands shown being myc: NORMAL CANCEROUS SKIN CELLS SKIN CELLS
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Name: ________________________________ (c, 6 pts) You perform a Southern blot and a Northern blot on normal breast cells and
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This note was uploaded on 01/23/2012 for the course BIOLOGY lsm1301 taught by Professor Seow during the Spring '11 term at National University of Singapore.

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2005_Exam4 - Name: _ 7.06 Cell Biology EXAM #4 May 18, 2005...

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