2005_pset1 - 7.06 Problem Set #1, Spring 2005 1. As a new...

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1 7.06 Problem Set #1, Spring 2005 1. As a new graduate student in a yeast lab, you decide to do a genetic screen looking for genes involved in mitosis, an essential biological process. After screening 1000 mutagenized haploid yeast strains at 23 o C and 36 o C, you find 20 strains that have defects in mitosis. These cells do not grow at the higher temperature but grow normally at the lower temperature. a. Why do you use haploid, and not diploid, yeasts for the screen? b. What is the name for the category of mutants that you have found? c. Why can’t you isolate mutants that have a defect in mitosis at all temperatures? d. How would you determine the total number of genes that are mutated in your collection of 20 mutant yeast strains? You decide to pursue your favorite gene from this screen, which you name YFG1 . You use genetic mapping techniques to figure out which gene in the yeast genome YFG1 is, and then you find the DNA sequence of YFG1 using the yeast genome database. You are now interested to see if YFG1 plays a role in mitosis in other organisms. e. How can you find out if YFG1 is present in the sequenced genomes of other organisms? f. A friend tells you that he thinks that it is very unlikely that a gene involved in mitosis in a single-celled microbe such as baker’s yeast would be present in the genome of other eukaryotes like plants and animals. Do you agree with your friend? You find a Drosophila (fruit fly) homolog of YFG1 called grounded . Flies with a mutation in this gene can’t fly and have tiny wings. The DNA sequence of this gene and a cDNA containing this gene are available to you. g. What is a simple experiment you could do to test if the grounded gene has a similar biological function as YFG1 ? You think the
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This note was uploaded on 01/23/2012 for the course BIOLOGY lsm1301 taught by Professor Seow during the Spring '11 term at National University of Singapore.

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2005_pset1 - 7.06 Problem Set #1, Spring 2005 1. As a new...

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