2005_pset8 - 7.06 Problem Set #8, Spring 2005 1. a. You...

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1 7.06 Problem Set #8, Spring 2005 1. a. You inject a significant amount of fluorescently labeled tubulin into a cell, and wait until all microtubules are labeled with fluorescence. You then use a laser to irradiate a small region of one of the microtubules, thereby bleaching the fluorescent dye so that it is no longer fluorescent. You then perform a timecourse in which you measure the recovery of fluorescence into the bleached area. For cells in interphase, you obtain the following data. Superimpose on this graph a drawing of the data you would expect to obtain if you repeated the experiment, but using cells proceeding through prophase. Explain your reasoning. b. Taxol is a drug that binds to and greatly stabilizes assembled microtubules; after taxol treatment, the microtubules are stable to all but the harshest denaturating conditions. This activity is in a sense the opposite of that seen with the drugs we discussed in class, like colchicines, which are microtubule-depolymerizing drugs. It turns out that both kinds of drugs – taxol and colchicines – are anti-cancer drugs. Why do you think that each of them works as an anti-cancer drug? 100% % of fluor. recovered time 60 min
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2 c. The frog oocyte (unfertilized egg) is a single cell that has asymmetry. The asymmetry of the frog oocyte is reflected in the asymmetric distribution of many mRNAs and proteins within the oocyte cytoplasm. The localization of one specific mRNA called Vg1 changes as the oocyte develops: - In early stage oocytes, Vg1 is found throughout the cytoplasm. - In intermediate stage oocytes, Vg1 is found traveling towards one end of the oocyte. - In late stage oocytes, Vg1 is only found at one end of the oocyte. Describe an experiment that shows that microtubules are necessary for the establishment of Vg1 mRNA localization to one end of the oocyte. d. Design an experiment that shows that microtubules are not necessary for the maintenance of Vg1 mRNA localization at one end of the oocyte. 2.
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This note was uploaded on 01/23/2012 for the course BIOLOGY lsm1301 taught by Professor Seow during the Spring '11 term at National University of Singapore.

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2005_pset8 - 7.06 Problem Set #8, Spring 2005 1. a. You...

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