7.06_2004_PS8 - 7.06 Spring 2004 PS 8 1 of 3 Problem Set 8...

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7.06 Spring 2004 PS 8 1 of 3 Problem Set 8 7.06 Cell Biology 1. a) The phosphorylation status of Rb regulates progression into S phase. Describe an experiment to show that the binding of Rb to E2F1 depends on the phosphorylation state of Rb. b) The Rb knockout mouse shows ectopic i.e. abnormal S-phase entry during embryogenesis and is not viable. What could explain this and how could you try to prove it? c) p53 is another tumor suppressor gene that is often deleted in human cancers. You have three cell lines (A, B, C) that were established from human thymic lymphomas. How would you investigate whether there is functional p53 in these cells? d) Cell lines A and B fail to show a rise in Bax protein levels following irradiation. You carry out a p53 western blot to check whether the defect is up or downstream of p53 induction. Cell line A shows induction of p53 upon irradiation. What could be responsible for the defect in induction of apoptosis in this cell line? e) Cell line B fails to show induction of p53 upon irradiation. While this explains the lack of Bax induction following irradiation, you are still curious as to what protein is mutant in these cells. List some possibilities and explain your reasoning. 2) You are an immunologist trying to uncover the molecular basis of T cell activation.
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7.06_2004_PS8 - 7.06 Spring 2004 PS 8 1 of 3 Problem Set 8...

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