Enzyme1 - Enzyme Action 1 (background for lab) What are...

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Enzyme Action 1 (background for lab) What are enzymes? proteins that catalyze biological reactions i.e., they make reactions go faster while not being consumed in the rxn How do enzymes work? enzyme + substrate ( s ) ! enzyme " substratecomplex ! enzyme + product ( s ) by binding to the substrate(s) and stabilizing the reaction intermediate enzymes lower the activation energy required to make a reaction proceed Why are enzymes so specific? Why do they only act on certain substrates? binding takes place in “active site”, a cleft in the protein which only fits the substrate sometimes similarly shaped molecules can fit into cleft--structure determines function How do we assay (measure) enzyme rate? by disappearance of a reactant or appearance of a product must have some reasonably simple way to measure amount of that substance expressed as change in concentration vs. time What affects reaction rate? the same things that affect the rate of any chemical reaction
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Enzyme1 - Enzyme Action 1 (background for lab) What are...

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