Unformatted text preview: -nnAAAA/TA/TA/TTA/TTTTnnAAAAnnn -A/T rich region -contacts the C-terminal of α-subunit of RNA poly Rho Utilization site = “rut” site (C-rich region) rRNA = made up of subunits -Dissociation of subunits can occur by decreasing conc. of Mg2+ in medium -motion of a particle in a centrifuge is defined by sedimentation coefficient -Svedberg, S -S increases with the MW of the sedimenting particle (shape also affects rate) -1 S = 10-13 sec -To separate 70S rRNA in E. coli – take whole cells, lyse, obtain 70S -70S separated by decreasing Mg2+ into 30S and 50S subunits -30S further separated into 16S + 21 different proteins, 50S further separated into 23S, 5S + 34 different proteins -S values are not additive (ie. 30S + 50S is not equal to 80S) loss of surface area, hence obtain 70S....
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This note was uploaded on 01/23/2012 for the course BCHM 311 taught by Professor Kelley during the Spring '09 term at University of Toronto.
- Spring '09