Testing the Tonicity of Potato Cells by the Addition of Sucrose

Testing the Tonicity of Potato Cells by the Addition of Sucrose

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The purpose of this experiment is to test the tonicity of potato cells. The potato cells are placed in six different Sucrose concentrations and left for an hour. The percent change in mass is then used to determine if the cells lost or gained water, therefore determining tonicity of the cell. The results show that the higher the concentration of sucrose the potatoes were placed in, the more hypotonic the potato becomes. The tonicity of a cell is the difference in osmotic concentrations between the cell and another solution or cell. The tonicity is determined by how concentrated each solution is on either side of a semipermeable membrane. When comparing solutions of different concentrations, the one that has more solute is known as hypertonic and the one with less solute is known as hypotonic. When the two solutions have equal concentrations, they are isotonic. (Biological Laboratories for Science Majors) When the potato cell becomes hypotonic to its surroundings (the sucrose solution), the potato cell will lose water and therefore shrivel. When the potato cell becomes hypertonic to its surroundings, the cell will gain water and turgor pressure will occur. Turgor pressure is the pressure exerted on the cell wall due to water uptake. (Campbell Biology) The loss or gain of water will result in a mass change of the potato cells, which will be able to be
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This note was uploaded on 01/24/2012 for the course BIOL 1208 taught by Professor Lee during the Fall '07 term at LSU.

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Testing the Tonicity of Potato Cells by the Addition of Sucrose

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