Systems Microbiolo23

Systems Microbiolo23 - the genome o Both approaches have...

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Systems Microbiology o Later people realized that you can use fluorescent labels instead of radiolabels. ° This meant that you didn’t have to deal with radioactivity ° It also meant that you could run them all in one lane. ° Instead of a slab gel, people use a thin tube, with a fluorescence detector automatically reading the wavelengths as they come out the other end. ° This method is fast and accurate o Graph: the progress of the Human Genome Project o Shotgun genome sequencing – what about the gaps? ° Doing a complete sequence for a bacterial genome is still expensive – around 10 or 20 thousand dollars, but it’s relatively straightforward. o Small insert cloning/sequencing o There are automated plate pickers now o Some people thought that shotgun sequencing would work (the Celera group used this) but others thought that it wouldn’t (the public consortium used a different method) o BAC sequencing – larger segments, you know where they lie on
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Unformatted text preview: the genome o Both approaches have advantages: shotgun sequencing is much quicker, but BAC sequencing closes the gaps the best method is to use some of both. o 454 Sequencing Commercial, massively parallel DNA sequencing technology: Run PCR with beads, each of which attaches to on strand of DNA Emulsion PCR Lots of DNA comes from an individual strand. You pour the reaction over a chip with tiny wells that each fit one bead. 1.5 million reactions all at once Thus the factory approach to sequencing is no longer necessary it can all be done in little machines like this. Its still pretty expensive, but its getting closer, and its changing things rapidly. o The evolution of sequencing is moving towards nano-methods, and pretty soon there will probably be sequencing technologies in hospitals....
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