Unformatted text preview: the genome o Both approaches have advantages: shotgun sequencing is much quicker, but BAC sequencing closes the gaps – the best method is to use some of both. o 454 Sequencing – Commercial, massively parallel DNA sequencing technology: ° Run PCR with beads, each of which attaches to on strand of DNA ° Emulsion PCR ° Lots of DNA comes from an individual strand. ° You pour the reaction over a chip with tiny wells that each fit one bead. ° 1.5 million reactions all at once ° Thus the factory approach to sequencing is no longer necessary – it can all be done in little machines like this. ° It’s still pretty expensive, but it’s getting closer, and it’s changing things rapidly. o The evolution of sequencing is moving towards nano-methods, and pretty soon there will probably be sequencing technologies in hospitals....
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- Fall '09
- GENOME SEQUENCING, DNA sequencing, Shotgun genome sequencing