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Unformatted text preview: the genome o Both approaches have advantages: shotgun sequencing is much quicker, but BAC sequencing closes the gaps the best method is to use some of both. o 454 Sequencing Commercial, massively parallel DNA sequencing technology: Run PCR with beads, each of which attaches to on strand of DNA Emulsion PCR Lots of DNA comes from an individual strand. You pour the reaction over a chip with tiny wells that each fit one bead. 1.5 million reactions all at once Thus the factory approach to sequencing is no longer necessary it can all be done in little machines like this. Its still pretty expensive, but its getting closer, and its changing things rapidly. o The evolution of sequencing is moving towards nano-methods, and pretty soon there will probably be sequencing technologies in hospitals....
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- Fall '09