Systems Microbiolo25

Systems Microbiolo25 - It encodes for a sex pilus, allowing...

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Systems Microbiology o Plasmids: ° Extrachromosomal DNA, usually circular (the distinction is a little fuzzy these days ° They encode the functions for the plasmid to replicate ° When plasmids are lost it’s generally because they fail to keep up with the cell’s replication ° There are high copy and low copy plasmids ° Resistance can be placed on plasmids ° Transposable elements and insertion elements ° Plasmids are a conveniently plastic entity, which helps explain how bacteria can get so resistant to antibiotics so quickly. ° F Plasmid • A lot of early studies made a lot of use of the F factor • It can incorporate itself into the cell’s chromosome, creating an F’ cell (the original origin of replication ends up in the middle)
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Unformatted text preview: It encodes for a sex pilus, allowing conjugation. F- F + Creation of an F strain Hfr Strains We can map out how long the gene transfer takes in minutes High resolution Mapping using Hfr strain Movie: bacteriophage T4 o Temperate phage and lysogeny o Phage conversion o In some cases phage make cells pathogenic o Specialized transduction (in phage lambda) Picks up genes that are flanking the pro-phage o Generalized transduction: sometimes the phage makes a mistake and packages the E. coli DNA instead of the phage DNA DNA transposition and transposons o Mobile genetic elements o Bacterial transposable elements o Transposon formation o Strategy for transposon mutagenesis o Mobile elements...
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