Unformatted text preview: • It encodes for a sex pilus, allowing conjugation. F-° F + • Creation of an F’ strain • Hfr Strains • We can map out how long the gene transfer takes in minutes • High resolution Mapping using Hfr strain ° Movie: bacteriophage T4 o Temperate phage and lysogeny o Phage conversion o In some cases phage make cells pathogenic o Specialized transduction (in phage lambda) ° Picks up genes that are flanking the pro-phage o Generalized transduction: sometimes the phage makes a mistake and packages the E. coli DNA instead of the phage DNA ° DNA transposition and transposons o Mobile genetic elements o Bacterial transposable elements o Transposon formation o Strategy for transposon mutagenesis o Mobile elements...
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This note was uploaded on 01/24/2012 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.
- Fall '09