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Unformatted text preview: These different methods incorporate the mother strands into the daughter strands in different ways. To determine this, they added 15 N labels experimentally they could predict the densities resulting from each replication method heavy vs. light The Conclusion, of course, was that it was semiconservative. o The replication process is not simple, and involves a lot of enzymes. Polymerase III cant just set down on a DNA strand it needs a DNA primer. That primer is then used to extend the DNA strand. This means that initially its not just a DNA strand its a DNA-RNA hybrid. The DNA polymerase synthesizes 5 3. This means that one strand goes more easily than the other strand. The leading strand goes directly, but the lagging strand develops a lot of smaller fragments these are called Okazaki fragments (about 1000 bases...
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- Fall '09