Systems Microbiolo30

Systems Microbiolo30 - ° These different methods...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
Systems Microbiology ° Slide: Central Dogma o Replication, Transcription, Translation o Reverse transcription sometimes ° Slide: Flow of information o Right now we’re just thinking about information flow within an individual species – how nucleic acid information flow occurs o How you maintain fidelity and copy your DNA for all your daughter cells ° What we know about DNA: o The four bases o The ring system of the sugar ° The number of carbons ° How you tell the difference between DNA and RNA (the lack of oxygen) o The nomenclature of the bases o Alternating linkage (3’ and 5’) o The base pairing (G-C, A-T) –This is how it replicates with such high fidelity. o Anti-parallel (5’ ° 3’, 3’ ° 5’) o When the structure was first determined, the pairing was inferred, but the method of replication wasn’t clear ° It could have been Semiconservative, Conservative, or Random Dispersive.
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: ° These different methods incorporate the mother strands into the daughter strands in different ways. ° To determine this, they added 15 N labels experimentally – they could predict the densities resulting from each replication method – heavy vs. light ° The Conclusion, of course, was that it was semiconservative. o The replication process is not simple, and involves a lot of enzymes. ° Polymerase III can’t just set down on a DNA strand – it needs a DNA primer. That primer is then used to extend the DNA strand. ° This means that initially it’s not just a DNA strand – it’s a DNA-RNA hybrid. ° The DNA polymerase synthesizes 5’ ° 3’. This means that one strand goes more easily than the other strand. The leading strand goes directly, but the lagging strand develops a lot of smaller fragments – these are called Okazaki fragments (about 1000 bases...
View Full Document

{[ snackBarMessage ]}

Ask a homework question - tutors are online