Systems Microbiolo30

Systems Microbiolo30 - These different methods incorporate...

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Systems Microbiology ° Slide: Central Dogma o Replication, Transcription, Translation o Reverse transcription sometimes ° Slide: Flow of information o Right now we’re just thinking about information flow within an individual species – how nucleic acid information flow occurs o How you maintain fidelity and copy your DNA for all your daughter cells ° What we know about DNA: o The four bases o The ring system of the sugar ° The number of carbons ° How you tell the difference between DNA and RNA (the lack of oxygen) o The nomenclature of the bases o Alternating linkage (3’ and 5’) o The base pairing (G-C, A-T) –This is how it replicates with such high fidelity. o Anti-parallel (5’ ° 3’, 3’ ° 5’) o When the structure was first determined, the pairing was inferred, but the method of replication wasn’t clear ° It could have been Semiconservative, Conservative, or Random Dispersive.
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Unformatted text preview: These different methods incorporate the mother strands into the daughter strands in different ways. To determine this, they added 15 N labels experimentally they could predict the densities resulting from each replication method heavy vs. light The Conclusion, of course, was that it was semiconservative. o The replication process is not simple, and involves a lot of enzymes. Polymerase III cant just set down on a DNA strand it needs a DNA primer. That primer is then used to extend the DNA strand. This means that initially its not just a DNA strand its a DNA-RNA hybrid. The DNA polymerase synthesizes 5 3. This means that one strand goes more easily than the other strand. The leading strand goes directly, but the lagging strand develops a lot of smaller fragments these are called Okazaki fragments (about 1000 bases...
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