Types of mutations

Types of mutations - Types of mutations, mutagenic agents,...

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Types of mutations, mutagenic agents, identifying mutants and mutagens = If a large number of cells from an antibiotic-sensitive culture are plated on agar medium containing the antibiotic, only antibiotic-resistant mutants will form colonies - More common are mutants that must be detected by indirect selection = An example of such a mutant is an auxotroph that, as a result of a mutation, has a requirement for a nutrient (e.g., an amino acid) that could be synthesized by its "parent" = Indirect selection of auxotrophs is made possible by replica plating (Tortora et al., Figure 8.21) · A culture exposed to a mutagen is diluted and spread onto "master plates" of nutrient-rich medium at a density of 100 cells per plate · The resulting colonies are picked up on a pad of sterile velvet, which is pressed onto "replica plates" of nutrient-rich and minimal media · Auxotrophs can be identified by the absence of particular colonies on the minimal medium replica Because many mutagens have been found to be
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This note was uploaded on 01/24/2012 for the course MCB MCB2010 taught by Professor Smith during the Fall '09 term at Broward College.

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