Lecture1mcb160Lf11

Lecture1mcb160Lf11 - MCB 160L Overview Forward genetics:...

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MCB 160L Overview Forward genetics : Define a phenotype and screen or select for mutations that generate that phenotype, starting with a random mutant collection. Goa l-Move from knowledge of the phenotype to identification of the gene. ( Drosophila -specific gene expression patterns; Arabidopsis -gametogenesis, embryonic phenotypes) .mutagen = mobile DNA elements. Reverse genetics : Start with a specific gene, identified at the molecular level. Goal- Create or identify mutations in that specific gene in order to study the phenotype produced when gene function is reduced or removed. ( C. elegans – RNAi to produce a phenocopy of mutation; S. cerevisia (yeast)– targeted knockout by homologous recombination) Mapping the location of genes: Analysis of segregation and linkage to determine chromosomal location; 3-pt cross; molecular and bioinformatic tools Molecular genetics tools: DNA isolation, restriction mapping and agarose gel electrophoresis, plasmids and bacterial transformation, subcloning and genetic engineering, PCR (as a tool for construction and analysis of DNA sequences, as a method to analyze genotypes obtained from small tissue samples, as a genomics tool)
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Use of Model Organisms
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Lecture1mcb160Lf11 - MCB 160L Overview Forward genetics:...

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