Lecture10f11.ppt

Lecture10f11.ppt - The frst three slides (aFter this one)...

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Unformatted text preview: The frst three slides (aFter this one) are From lecture 5 and 6 and will be used to remind you where we are in Dros Exp. 1. The overall goal of Drosophila Experiment 1 is to generate new mutations In the course of the Drosophila experiments you will (1) recover new mutations- using a transposable element (P-element, p[w+}) as the mutagen, to create new insertions on the autosomes X II or III (2) Look for interesting gene expression patterns of genes located near the new transposon locations ( enhancer trap ) As part of Drosophila Experiment 2 (a logical extension of Exp. 1) (3) carry out an exercise illustrating how you would determine what chromosome the P-element moved to, and an experiment to determine the map position of a P element segregation analysis and mapping by 3 point cross (the segregation will be done in a computer exercise, the 3pt cross will be with existing insertions due to time constraints =Dros. Exp. 2) (4) Determine whether insertion of the P-element fully or partially inactivated a gene, resulting in a phenotype when the P-element-containing chromosome is made homozygous, and identify the location of the P-element in the genome. (This will also be done as a wirtten and computer exercise). Cross 1.1 a-c (Experimental) Purpose: Generate progeny with transpositions of p[w+] from X onto autosomes Females y w ; + ; + ; + x F1 Sb males (provided) y w + + + Cross 1.1d Control) Females y w ; + ; + ; + x F1 normal bristle males (provided) y w + + + In lab 2 you set up: Cross 1.0 Purpose is to place transposase source p[ 2-3] (from father) and movable p[w+] element (from mother) in the same F1 genome Females w p[w+ Gal4] ; + ; + ; + x...
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Lecture10f11.ppt - The frst three slides (aFter this one)...

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