Lecture12mcb160L+f11revised

Lecture12mcb160L+f11revised - KanS (Sensitive) KanR...

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Kan S (Sensitive) Kan R (Resistant)
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Screen for Gametophyte /Embryo Mutants Ds + plants Score progeny for Kan R : Kan S X Ds Gene disruption KAN R Kan R Gene X 1. No effect on embryo/gametophytes 3:1 2. Embryo Lethal 2:1 3. òP r ñ Gametophyte Lethal 1:1 Kan R :Kan S
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Screen for Gametophyte /Embryo Mutants Ds + plants Score progeny for Kan R : Kan S X Ds Gene disruption KAN R Kan R Gene X 1. No effect on embryo/gametophytes 3:1 2. Embryo Lethal 2:1 3. òP r ñ Gametophyte Lethal 1:1 4. Partial lethal for òP r ñ Gametophyte <2:1 but >1:1 e.g. 1.5:1 5. Affects both Gametophytes <1:1 0.5:1 6. No effect, and parent is homozygous 1:0 Kan R :Kan S
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Enhancer Trap Enhancer TATA Reporter gene Gene Trap Exon SA Reporter gene Reporter gene: lacZ (animal) or GUS (plant) or GFP (both animals and plants) Transposons can be used to identify genes by “trapping” A “trap” is an element that captures the expression pattern of a gene
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Gene Trap/ Enhancer Trap Transposons 1. Identifcation oF genes by patterns oF expression e.g.
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This note was uploaded on 01/25/2012 for the course MCB 160L160L taught by Professor Venkatesansundaresan during the Fall '11 term at UC Davis.

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Lecture12mcb160L+f11revised - KanS (Sensitive) KanR...

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