Lecture14-ReverseGenetics%2C+C.+elegans

Lecture14-ReverseGenetics%2C+C.+elegans - epistasis:...

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Unformatted text preview: epistasis: analyze phenotype of double mutant how can you use this information to order gene function in a pathway? If genes positively interact, the epistatic gene functions upstream If genes negatively interact, the epistatic gene functions downstream eye color in Drosophila E-maps h"p://idekerlab.ucsd.edu/research/Pages/ Diferen7alNetworks.aspx haploid ade auxotrophs Yeast experiment 1: ade2 ade3 mating plate: Yeast experiment 1: medium lacking adenine: Yeast experiment 1: tetrad spore colonies Yeast experiment 1: replica plating Yeast experiment 1: h"p://en.wikipedia.org/wiki/Replica_pla7ng analysis of tetrads: Infer genotype from phenotype Determine epistatic gene Determine pathway for adenine biosynthesis Yeast experiment 1: Exploiting Spore-Autonomous Fluorescent Protein Expression to Quantify Meiotic Chromosome Behaviors in Saccharomyces cerevisiae h"p://www.gene7cs.org/content/current Saccharomyces cerevisiae genome 16 chromosomes- ~12 Mb DNA very little repetitive DNA ~6000 genes genes typically have no introns, a few hundred genes have one or two introns h"p://www.yeastgenome.org/ Reverse genetics - generating knockouts...
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Lecture14-ReverseGenetics%2C+C.+elegans - epistasis:...

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