Lecture17f11

Lecture17f11 - Replication Cohesin proteins keeps sister...

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Unformatted text preview: Replication Cohesin proteins keeps sister chromatids together Homolog Pairing and Crossing Over Homologs pair and synaptonemal complex (SC) forms. The SC and other proteins are necessary for recombination, formation of chiasmata Meiosis I- Homologs pair,synapse and undergo recombination Chaismata ensure proper homologous chromosome segregation Chromosome alignment and segregation Chiasmata (the remnants of recombination) ensure that homologs connect to opposite spindle poles, so that one homolog is segregated to each daughter cell SC bivalent WT bivalent SC proteins Syp-1 mutant: univalents Lack of SC proteins C. Elegans RNAi Experiment C. elegans report- see posted instructions-Assume that we know that syp-1 is a component of the synaptonemal complex RNAi- produces univalents when chromosomes are scored- many dead embryos and increased frequency of males among survivors The experiment is to test the role of WT bivalent SC proteins Syp-1 mutant: univalents Lack of SC proteins C. Elegans RNAi Experiment C. elegans report- see posted instructions-Assume that we know that syp-1 is a component of the synaptonemal complex RNAi- produces univalents when chromosomes are scored- many dead embryos and increased frequency of males among survivors The experiment is to test the role of syp-2 The overall goal of Drosophila Experiment 1 is to generate new mutations In the course of the Drosophila experiments you will (1) recover new mutations- using a transposable element (P-element, p[w+}) as the mutagen, to create new insertions on the autosomes X II or III (2) Look for interesting gene expression patterns of genes located near the new transposon locations ( l enhancer trap z ) As part of Drosophila Experiment 2 (a logical extension of Exp. 1) (3) carry out an exercise illustrating how you would determine what chromosome the P-element moved to, and an experiment to determine the map position of a P element – segregation analysis and mapping by 3 point cross (the segregation will be done in a computer exercise, the 3pt cross will be with existing insertions due to time constraints =Dros. Exp. 2) (4) Determine whether insertion of the P-element fully or partially inactivated a gene, resulting in a phenotype when the P-element-containing chromosome is made homozygous, and identify the location of the P-element in the genome. (This will also be done as a wirtten and computer exercise). In a real screen (ie without our time limitations) we would have frst crossed our exceptional males to yw virgins to generate more progeny For each line with the new insertion....
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This note was uploaded on 01/25/2012 for the course MCB 160L160L taught by Professor Venkatesansundaresan during the Fall '11 term at UC Davis.

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Lecture17f11 - Replication Cohesin proteins keeps sister...

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