Lecture18RAD1

Lecture18RAD1 - 481 kertlkkkkr kltltqmvgk peeleeegdv...

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genetic change & disease
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autosomal recessive genetic disorder defective in DNA repair extremely sensitive to UV light predispositon to cancer Xeroderma pigmentosum “XP”
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Xeroderma pigmentosum “XP” http://www.ncbi.nlm.nih.gov/books/NBK1397/
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XPF ERCC4 = structure specifc endonuclease involved in NER
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thymine dimers
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H. sapiens why we don’t want to study XP in humans
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1 mesgqparri amaplleyer qlvlelldtd glvvcarglg adrllyhflq lhchpaclvl 61 vlntqpaeee yfinqlkieg vehlprrvtn eitsnsryev ytqggvifat srilvvdflt 121 dripsdlitg ilvyrahrii escqeafilr lfrqknkrgf ikaftdnava fdtgfchver 181 vmrnlfvrkl ylwprfhvav nsfleqhkpe vveihvsmtp tmlaiqtail dilnaclkel 241 kchnpsleve dlslenaigk pfdktirhyl dplwhqlgak tkslvqdlki lrtllqylsq 301 ydcvtflnll eslratekaf gqnsgwlfld sstsmfinar arvyhlpdak mskkekisek 361 meikegeetk kelvlesnpk wealtevlke ieaenkesea lggpgqvlic asddrtcsql 421 rdyitlgaea fllrlyrktf ekdskaeevw mkfrkedssk rirkshkrpk dpqnkerast
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Unformatted text preview: 481 kertlkkkkr kltltqmvgk peeleeegdv eegyrreiss spescpeeik heefdvnlss 541 daafgilkep ltiihpllgc sdpyaltrvl hevepryvvl ydaeltfvrq leiyrasrpg 601 kplrvyfliy ggsteeqryl talrkekeaf eklirekasm vvpeeregrd etnldlvrgt 661 asadvstdtr kaggqeqngt qqsivvdmre frselpslih rrgidiepvt levgdyiltp 721 emcverksis dligslnngr lysqcismsr yykrpvllie fdpskpfslt srgalfqeis 781 sndisskltl ltlhfprlri lwcpsphata elfeelkqsk pqpdaatala itadsetlpe 841 sekynpgpqd fllkmpgvna kncrslmhhv kniaelaals qdeltsilgn aanakqlydf 901 ihtsfaevvs kgkgkk protein sequence of ERCC4 identify yeast ortholog yeast RAD1 + + + + Amplifcation oF Rad-His cassette via PCR From plasmid template TransFormation Host yeast strain is his3 (deletion) and is thereFore an auxotroph (cannot grow on minimal plates - histidine). Yeast experiment 2:...
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Lecture18RAD1 - 481 kertlkkkkr kltltqmvgk peeleeegdv...

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