Lecture19post

Lecture19post - The overall goal of Drosophila Experiment 1...

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The overall goal of Drosophila Experiment 1 is to generate new mutations In the course of the Drosophila experiments you will (1) recover new mutations- using a transposable element (P-element, p[w+}) as the mutagen, to create new insertions on the autosomes X II or III (2) Look for interesting gene expression patterns of genes located near the new transposon locations ( l enhancer trap z ) As part of Drosophila Experiment 2 (a logical extension of Exp. 1) (3) carry out an exercise illustrating how you would determine what chromosome the P-element moved to, and an experiment to determine the map position of a P element – segregation analysis and mapping by 3 point cross (the segregation will be done in a computer exercise, the 3pt cross will be with existing insertions due to time constraints =Dros. Exp. 2) (4) Determine whether insertion of the P-element fully or partially inactivated a gene, resulting in a phenotype when the P-element-containing chromosome is made homozygous, and identify the location of the P-element in the genome. (This will also be done as a wirtten and computer exercise).
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Identify the location of the P-element in the genome Transposable element mutagenesis “tags” the DNA- provides a means to identify the genomic DNA flanking the insertion site of the transposon and thus identify the gene that has been “trapped” when an enhancer or gene trap construct is used.
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This note was uploaded on 01/25/2012 for the course MCB 160L160L taught by Professor Venkatesansundaresan during the Fall '11 term at UC Davis.

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Lecture19post - The overall goal of Drosophila Experiment 1...

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