MCB160L Datarecord and worksheets

MCB160L Datarecord - MCB 160L Fall 2011 Data Records and Worksheets The file contains the DataRecords and Worksheets from the fall 2011 version of

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1 MCB 160L Fall 2011 Data Records and Worksheets The file contains the DataRecords and Worksheets from the fall 2011 version of the manual, in case you need a clean copy of any of them. Note that some assignments, such as the Drosophila and second written reports are not included. They will posted separately to the website. Copyright © UC Regents Davis campus, 2005-11. All Rights Reserved. Contents may not be re-distributed without prior written consent of course instructor.
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D-17 Data Record Sheet D1 (Drosophila Expt 1) Name ____________________ 1. From your Cross 1.0 bottle, obtain the males and count progeny as instructed in the lab manual. Count a random sample of about 100 flies, scoring for the phenotypes listed below. Keep track of the numbers in your notebook and then transfer to the chart below when complete. a. Separate by sex, and then for each sex, separate and count the Sb and non-Sb flies. b. Within each group of flies, separate into four possible classes, recording the number of each (note that only some types may be present in each of groups): i. No evidence of somatic transposition (original uniform p[w+] eye color) ii. Variegated eyes iii. Completely white eye - note if this is unilateral (U, one eye only) or bilateral (B, both eyes). iv. Completely red eye (darker than original p[w+] stock) - note if this unilateral or bilateral. c. Compare the eye color of non-variegated males and non-variegated females and note the difference. (There is a similar difference between the variegated males and females but the patchiness makes it harder to detect.) 3. Fill charts using numbers, not hash marks. Remember to include the male flies you used for your crosses in your final numbers. SUMMARY of TOTAL NUMBERS: non-Sb Sb Male Female Non-variegated orange eyes Variegated eyes Completely white eye (U / B) Completely red eye (U / B) Sb male Sb female Wild type (non- Sb) male Wild type (non- Sb) female
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D-18 Worksheet D1 (Drosophila Expt 1) NAME In addition to your data, use the manual and your lecture notes to answer questions on this and all Datarecord/Worksheets to come. 1. Compare the total number of males and females in the progeny of Cross 1.0. Is this number consistent with Mendelian expectations? Write down the expectations (how many males and females should you have seen) and compare them to your data in your answer. Are your own numbers more or less consistent than the class data provided? (Note you do not need to do statistical analysis here, but rather just a qualitative comparison. 2. Is the total number of Stubble and non- Stubble progeny of each sex consistent with Mendelian expectations? Write down the expectations (how many Stubble and non-Stubble progeny should you have seen) and compare them to your data in your answer. Are your own numbers more or less consistent than the class data? (Note you do not need to do statistical analysis here, but rather just a qualitative comparison). 3. Is there any correlation between the eye color and bristle morphology? Explain this finding and
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This note was uploaded on 01/25/2012 for the course MCB 160L160L taught by Professor Venkatesansundaresan during the Fall '11 term at UC Davis.

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MCB160L Datarecord - MCB 160L Fall 2011 Data Records and Worksheets The file contains the DataRecords and Worksheets from the fall 2011 version of

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