MCB160L Exercises Lab1_2011

MCB160L Exercises Lab1_2011 - 1 MCB 160L Fall 2011 Lectures...

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Unformatted text preview: 1 MCB 160L Fall 2011 Lectures MW 1:10-2 in 1344 Storer Sec. 02 MW 2:10-5 pm - Dr. L. Rose Sec. 10 TuTh 2:10-5 pm Drs. V. Sundaresan and J. Engebrecht Lab sections are in 3060 SLB TABLE OF CONTENTS LECTURE/LAB SCHEDULE ...................................................................................................... 2 MCB Course Materials Fee Information ..................................................................................... 5 Modules Drosophila: Introduction, Genetic notation, variations, and morphology .................................. D-1 Exp.1: Creation of new P element insertions.......................................................... D-9 Screening for GFP patterns ....................................................................... D-21 Exp.2: Recombination mapping of an existing P element .................................... D-31 Molecular Biology: Introduction.............................................................................................................. M-1 Exp.1: Agarose gel electrophoresis ....................................................................... M-10 Exp.2: Restriction enzyme mapping of plasmid DNA ........................................... M-24 Exp.3: Transformation of E. coli ........................................................................... M-29 Exp.4: Subcloning into plasmid vectors.................................................................. M-34 Exp.5: PCR amplification of human VNTR sequences .......................................... M-57 Arabidopsis Introduction and Exp. 1: Screening for embryo and gametophyte mutants using transposons ...................... A-1 Yeast Introduction Exp. 1: Complementation and Epistasis.............................................. Y-1 Exp. 2: Knockout of a yeast gene ............................................................................ Y-5 C. elegans Introduction, Genetics, overview of biology, mutants............................................. C-1 Exp. 1: RNA interference......................................................................................... C-7 NOTE: The yllabus will be handed out in class and posted on the class SmartSite. Additional material for completing exercises and reports will also be posted on the class website, but most worksheets and other lab assignments are found at the end of each Experiment within each module. Copyright UC Regents Davis campus, 2005-11. All Rights Reserved. Contents may not be re-distributed without prior written consent of course instructor. 2 MCB 160L PRINCIPLES OF GENETICS LABORATORY Spring 2010 Lab Schedule- NOTE these are just general reminders- refer to manual for details Week/ Lab Date Molecular Experiments Drosophila Experiments C. elegans Experiments Yeast Experiments Arabidopsis Experiments Lecture Topics (instructor) Week1 #1 Sept M26 T27 Intro to Molecular Biology (Mi.1-i.3): pipetman use and accurate measurement Introduction to Drosophila Course info, Intro to Drosophila...
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MCB160L Exercises Lab1_2011 - 1 MCB 160L Fall 2011 Lectures...

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