MCB160LMT3practicefl1_1_

MCB160LMT3practicefl1_1_ - NAME:_ Section:_ ID#(last 4...

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
NAME:__________________________________ Section:__________________________________ ID#(last 4 digits)__________________________ MCB160L- EXAM 3 Practice Fall 2011 PLEASE WRITE YOUR NAME ON THE TOP OF ALL PAGES, BUT DO NOT START THE EXAM UNTIL INSTRUCTED.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Name: __________________________ p. 2 Question 1 ( 7pts) a) (3pts) Write out the following steps (A-E) of meiosis in the correct order. Write the letters from left to right, with the leftmost letter being the first event, and arrows showing the order (.e.g A-> B- >…means A comes first, then B) A) Synapsis B) Segregation of homologous chromosomes C) Segregation of sister chromatids D) DNA replication E) Recombination/formation of chiasmata b) (1pt) Write the letter of the FIRST step from the list above that a syp-1 or syp-2 RNAi treated worm would exhibit defects in ____________ c) (1pt) The trigger for RNAi that is introduced into worms by feeding is (Circle the BEST answer): a) Single stranded RNA b) Double stranded RNA c) Single stranded DNA d) Double stranded DNA d) (1pt) Once the trigger is introduced, it causes a loss of gene function through a mechanism that specifically acts to (Circle the BEST answer): a) Block transcription of the target gene b) Cleave target mRNA molecules and thus degrade them c) Cleave target protein molecules and thus degrade them d) Alter protein activity e) (1pt) In the experiment you did in lab, worms that grew up on syp-2 RNAi plates became adults with the Syp phenotype: they had oocytes with univalents and produced many dead embryos. Some embryos hatched however and these grew up to the adult stage on regular bacterial plates (no RNAi). If you now examined the phenotype of these worms, you would expect them to (Circle the BEST answer): a) All show the same syp-2 phenotype as the 1 st generation on syp-2 plates b) All show wild-type meiosis, i.e. none show the syp-2 phenotype c) Segregate 3:1 for the syp-2 and wild-type phenotypes d) Show an even stronger syp-2 phenotype with each generation.
Background image of page 2
Name: __________________________ p. 3 Question 2. (6 pts) To begin to map you p[w+] insertions, you would cross non-white (orange-eyed) males from each new line to females containing the CyO balancer and also to females containing the TM3 Sb balancer as follows: males w/Y; p[w+] autosomal /+ x virgin females w/w; CyO/Sp; +/+;+/+ and w/w; +/+; TM3 Sb/Gl; +/+ a) (4pts) Assume that the p[w+] is on chromosome II . You take 3 orange TM3 Sb males progeny from cross above and mate them to 3 virgin females that are w/w; +/+; +/+ ; +/+ in the chromosome 3 segregation cross. Which one
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 9

MCB160LMT3practicefl1_1_ - NAME:_ Section:_ ID#(last 4...

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online