Practice Exam1 f11

Practice Exam1 f11 - TOPICS TO STUDY FOR MIDTERM#1 MCB 160L...

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TOPICS TO STUDY FOR MIDTERM #1 – MCB 160L Fall 2011 Midterm Wednesday Oct. 19 Covers Lecture and lab material for 1- 6, including Worksheets/Data Record Sheets turned in through Monday Oct. 17. This list includes a reminder of the major topics we have covered …you are responsible for other subtopics also covered in these lectures but not explicitly listed here. Similarly, keep in mind that the practice test posted gives an idea of the length and type of question, but is not all inclusive in terms of topics. Note also that although there will not be detailed questions on the general background covered at the beginning (e.g. forward genetics, Drosophila life cycle, genetic conventions), these topocs are listed because you need to have a working knowledge of these aspects to understand the experiments and concepts. Meaning of forward genetics, reverse genetics Basic Drosophila life cycle Genetic conventions for naming mutations and genotypes, characteristics of balancer chromosomes P-elements: structure, properties, types (wild type vs. engineered lab varieties), transposase (wild- type and engineered) Factors that can affect expression level of w+ gene on a P-element (and therefore eye color) Effect of P-element mobilization on germline and somatic cells (how this results in variegated eyes and new germline mutations). Purpose and outcomes of Cross 1.0 Be able to predict the resulting genotypes and phenotypes in the progeny. Why you select males with Sb from Cross 1.0 progeny to set up Cross. 1.1. Understand the purpose of Cross 1.1, but you do not need to predict progeny for this one. Calculations needed to make specific solutions starting from solid chemicals or known stock solutions Calculations to determine the concentration of bacteria in a stock (working with serial dilutions) Properties and characteristics of restriction enzymes and plasmids Theory of electrophoretic separation of DNA fragments Effect of fragment size Effect of DNA topology Visualization by Ethidium Bromide staining Calculation of size of unknown fragment based on size marker migration Basic principles of restriction mapping Theory of DNA transformation into competent cells Ligase: Uses, Structure of nick that is substrate for ligase (3’OH, 5’P) Ligation theory and practice: Parameters that affect the outcome of ligation reactions (competing reactions, relative concentrations of ends…do not need to memorize the equation), how to minimize recircularization, phosphatase, directional cloning vs. non-directional cloning Common features of plasmids (origin, selectable marker, MCS or polylinker (Will NOT cover blue/white screening details, expression vector/reading frame considerations- that will be covered later after you have seen it in lab.) Bacterial transformation calculations as on worksheet 6 (not transformation mechanism-already
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This note was uploaded on 01/25/2012 for the course MCB 160L160L taught by Professor Venkatesansundaresan during the Fall '11 term at UC Davis.

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Practice Exam1 f11 - TOPICS TO STUDY FOR MIDTERM#1 MCB 160L...

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