Lab 4 - Lindle 1 J. Michael Lindle Mr. O AP Bio 30 Nov....

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Lindle 1 J. Michael Lindle Mr. O AP Bio 30 Nov. 2009 Lab Four: Plant Pigments and Photosynthesis Tables: Table 4.1 Spinach Distance Moved by Pigment Band (millimeters) Band Number Distance (mm) Band Color 1. 10 Light yellow 2. 47 Greenish yellow 3. 71 Green 4. 82 Bright yellow Distance Solevent Front Moved 86 (mm) Ridiccio Distance Moved by Pigment Band (mm) Band Number Distance (mm) Band Color 1. 8 Purple Distance Solvent Front Moved 82 (mm) Table 4.2 Table 4.3 Cuvettes 1 Blank (No DPIP) 2 Unboiled Chloroplasts Dark 3 Unboiled Chloroplasts Light 4 Boiled Chloroplasts Light 5 No Chloro- plasts Light Phosphate Buf- fer 1 mL 1 mL 1 mL 1 mL 1 mL Distilled H2O 4 mL 3 mL 3 mL 1 mL 3 mL+3 drops DPIP - 1 mL 1 mL 1 mL 1 mL Unboiled Chloroplasts 3 drops 3 drops 3 drops - - Boiled Chloro- plasts - - - 3 drops - Table 4.4
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Lindle 2 Transmittance (%) (My Group) Times are in minutes Cuvette 0 min. 5 min. 10 min. 16 min. 2 Unboiled/Dark 22 23.8 24.8 26.2 3 Unboiled/Light 23.4 24.2 23.8 23.2 4 Boiled/Light 27.2 28.6 27.8 27.6 5 No Chloroplasts/ Light 25.4 20 25.2 24.8 Transmittance (%) (Kevin’s Group) Times are in minutes Cuvette 0 min. 8:22 min. 15:52 min. 22:20 min. 2 Unboiled/Dark 20 21.6 20.4 19.4 3 Unboiled/Light 18.2 25.6 31.4 36.4 4 Boiled/Light 17.8 18.0 17.8 17.6 5 No Chloroplasts/ Light 13.4 13.2 13.0 12.8 Graph: This graph uses Kevin’s Group Data The Independent Variable: Time (minutes) The Dependent Variable: Transmittance (%) Changes in Transmittance Over Time J Tr an s mi tta nc e ( % ) Time (In Minutes)
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Lindle 3 Questions: 4A: 1. The differences in solubility of the pigments and the attraction of the solvents to the paper are involved in the separation of the pigments. The carotene does not form hy- drogen bonds with the cellulose and it is very soluble in the solvent giving it the ability to move the farthest. Because xanthophyll has oxygen in it, it bond to the cellulose through Hydrogen bonding. Xanthophyll moves less than carotene because it bonds with the cellulose and it is less soluble in the solvent. Chlorophyll a and b both contain oxygen and nitrogen, therefore they bond to the cellulose of the paper. Chlorophyll a
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This note was uploaded on 01/25/2012 for the course BIO 1111 taught by Professor Odolecki during the Fall '10 term at GWU.

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Lab 4 - Lindle 1 J. Michael Lindle Mr. O AP Bio 30 Nov....

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