J. Michael Lindle
30 Nov. 2009
Temperature effects the rate of cellular respiration.
3 fish tanks, 3 bony fish (must be the same type of fish with similar dimen-
sions), 1 stop watch, 3 people counting.
* (choose your animal) The two fish that you choose must be of the same kind
and must fit under the category of Osteichthyes (bony fish). Trout, salmon, ocean sun-
fish, oarfish, eels, Atlantic blue marlon, giant grouper, and the dwarf pygmy goby should
work fine. I chose the Rainbow Trout.
It is a fact that the lower the water temperature is, the higher
amount of dissolved oxygen is in the body of water. As expected, the warmer the body
of water is, the less dissolved oxygen is in it.
Hypothesis (results expected):
Every time a bony fish needs to breathe, the Operculum
opens (bony flap over gills) and gas exchange occurs. In cold water (10°C), the Rain-
bow Trout is likely to open it’s Operculum less often because each time it takes in more
oxygen since there is a higher concentration of dissolved oxygen in the water. In warm-
er water (22°C) the Rainbow Trout is likely to open it’s Operculum more often because
each time it takes in less oxygen since there is a lower concentration of dissolved oxy-
gen in the water. The fish in the 15°C water should have an Operculum flapping rate
that fits in between the other two fish; we’ll call that average.
Procedure (design an experiment):
1. Fill the first tank with water that is 10°C, fill the second tank with water that is 15°C,
and fill the third tank with water that is 22°C. Make sure to put the same amount of
water in each tank.
2. Add the Rainbow Trout to the tanks and allow them to settle in for a couple of minutes
as you gather your two partners and your stop watch.
3. Next you will start the stop watch and immediately after count how many times the
Operculum opens. You will record your data every 30 seconds for 4 minutes; always
counting from where you left off. For example, after 30 seconds you counted 25, at 31
seconds after the flap opens again, you will continue to say 26, 27, etc…
4. Record your data, fire up the grill, and dig in.
Quantify Results Derived Unit:
Say for example one of the Rainbow Trout takes 330 flaps of breath every 5 minutes.
That reduces down to 11 flaps of breath every 10 seconds. The rate of respiration in this
Trout’s case is 1.1 flaps/1 second.