2009-Chang_et_al - Genetic variation and phylogenetics of...

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and Y. T. Ju W. H. Chang, H. P. Chu, Y. N. Jiang, S. H. Li, Y. Wang, C. H. Chen, K. J. Chen, C. Y. Lin analyzed by nineteen microsatellite markers Genetic variation and phylogenetics of Lanyu and exotic pig breeds in Taiwan doi: 10.2527/jas.2007-0562 originally published online Aug 15, 2008; 2009.87:1-8. J Anim Sci http://jas.fass.org/cgi/content/full/87/1/1 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by on October 22, 2010. jas.fass.org Downloaded from
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ABSTRACT: The Lanyu pig is an indigenous min- iature pig breed on Lanyu Islet near Taiwan, with a mitochondrial DNA genetic lineage remote from Asian and European pig breeds. The unknown population genetic structure and increased inbreeding among the small population of conserved Lanyu pigs is now of great conservation concern. Additionally, the presence for more than a century of exotic pig breeds in Taiwan has made gene introgression from exotic pig breeds into Lanyu pigs very possible. The present study thus aimed to investigate nuclear genetic variation within the con- served Lanyu pigs and the phylogenetic relationship and possible genetic introgression between Lanyu and exotic pig breeds by determining the polymorphism of 19 microsatellite loci. In the neighbor-joining tree con- structed from 7 pig breeds based on Cavalli-Sforza and Edward chord genetic distances, 3 major clades were recognized, in which the Asian and European breeds were separately clustered into 2 clades with a 93.0 and 99.9% bootstrap confidence value, respectively. All in- dividuals of the Lanyu breed formed a unique subclade within the Asian clade based on the distance of the pro- portion of shared alleles, −ln(ps), suggesting that the Lanyu breed possesses a unique nuclear genetic struc- ture and that no nuclear gene introgression from exotic breeds into the conserved Lanyu pigs has occurred in recent history. Fifteen of 19 microsatellite loci devi- ated from Hardy-Weinberg equilibrium (by Wright’s statistic), suggesting a significant loss of heterozygos- ity in the conserved population. The valuable nuclear genetic structure and phylogenetic information should assist future conservation and population management of Lanyu pigs. Key words: genetic variation, introgression, Lanyu breed, microsatellite, phylogenetics, pig ©2009 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2009. 87:1–8 doi:10.2527/jas.2007-0562 INTRODUCTION The Lanyu pig is an indigenous miniature pig breed from Lanyu Islet, off southeast Taiwan, with a char- acteristic small body, narrow and straight head, small erect ears, and coarse black hair. Its phenotype dif- fers from the domestic Asian and European pig breeds (Cheng, 1986). For the conservation of Lanyu pigs and to develop a laboratory pig stock, 2 herds of Lanyu pigs were moved from Lanyu Islet to Taiwan, to the National Taiwan University ( NTU ) teaching farm in 1975, and to the Taitung Animal Propagation Station ( TAPS ) in 1980 (Chyr et al., 2001). Both herds were allowed panmixia from 1975 to 2004. Only 2 mitochon-
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This note was uploaded on 01/26/2012 for the course ECON 2272 taught by Professor Gay during the Spring '08 term at Birmingham-Southern College.

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2009-Chang_et_al - Genetic variation and phylogenetics of...

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