2009-Duangjinda_et_al - Detection of bovine leukocyte...

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Chaiyotvittayakul M. Duangjinda, D. Buayai, V. Pattarajinda, Y. Phasuk, S. Katawatin, T. Vongpralub and A. mastitis resistance in Holstein x Zebu Detection of bovine leukocyte antigen DRB3 alleles as candidate markers for clinical doi: 10.2527/jas.2007-0789 originally published online Sep 26, 2008; 2009.87:469-476. J Anim Sci http://jas.fass.org/cgi/content/full/87/2/469 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by on February 2, 2011. jas.fass.org Downloaded from
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ABSTRACT: Bovine leukocyte antigen DRB3 alleles from Holstein × Zebu crossbred dairy cows (n = 409) were analyzed using the PCR-RFLP technique. Exon II of DRB3 was amplified using locus-specific primers (HLO30/HLO32), followed by digestion with 3 restric- tion enzymes ( Rsa I, Bsty I, and Hae III). Forty alleles were found with frequency ranging from 0.005 to 0.139. The most frequently detected alleles of Holstein × Zebu were DRB3*16, *51, *23, *11, *8, and *1, accounting for 61.12% of the alleles in the population. Detection of candidate alleles for clinical mastitis occurrence was performed by logistic regression. It was found that per- centage of Holstein fraction in crossbred cows had a nonsignificant effect ( P > 0.05). However, parity had a significant effect on mastitis occurrence. In addition, DRB3*1 and *52 were the most associated with the oc- currence of clinical mastitis, whereas *15, *51, and *22 were associated with resistance in crossbred popula- tions. This is the first report of association of DRB3*15 and *51 with mastitis resistance. The association was validated by examining the candidate alleles in another commercial population. Highly susceptible (n = 43) and resistant (n = 42) groups of Holstein × Zebu cows were investigated. The result confirmed that DRB3*1 and *52 could be considered as susceptibility alleles, whereas *15, *51, and *22 could be considered as re- sistant alleles in Holstein × Zebu raised under tropi- cal conditions. In addition, allele effects on 305-d milk production were estimated by BLUP. It was shown that most alleles associated with high clinical mastitis occur- rence were related to increased milk yield. This study revealed that allele DRB3*10 had the greatest effect on increasing milk yield with moderate resistance to clini- cal mastitis, which could be used as a potential marker for selection in dairy genetic evaluation. Key words: bovine leukocyte antigen DRB3 allele, candidate marker, clinical mastitis, Holstein × Zebu ©2009 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2009. 87:469–476 doi:10.2527/jas.2007-0789 INTRODUCTION Crossbreeding between Holstein Friesian and Zebu with different levels of Holstein fraction has been used for dairy production in many tropical countries, such as Thailand, Vietnam, and the Philippines. Selection of dairy cows for mastitis resistance is a challenging con- cept for dairy producers and breeders, because mastitis is one of the most common diseases in dairy produc- tion. The incorporation of molecular genetic markers in the selection process through marker-assisted selection
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This note was uploaded on 01/26/2012 for the course ECON 2272 taught by Professor Gay during the Spring '08 term at Birmingham-Southern College.

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2009-Duangjinda_et_al - Detection of bovine leukocyte...

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