{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

2009-Kuehn_et_al - Genetic relationships of body...

Info icon This preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
L. A. Kuehn, D. J. Nonneman, J. M. Klindt and T. H. Wise Genetic relationships of body composition, serum leptin, and age at puberty in gilts doi: 10.2527/jas.2008-0936 originally published online Oct 10, 2008; 2009.87:477-483. J Anim Sci http://jas.fass.org/cgi/content/full/87/2/477 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by on February 2, 2011. jas.fass.org Downloaded from
Image of page 1

Info icon This preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
ABSTRACT: Leptin produced by adipocytes acts through leptin receptors in the hypothalamus to con- trol appetite and food intake and thus communicates information about degree of fatness. It is thought that a degree of body fat is required for initiation of pu- berty and maintenance of reproductive function in mammals. The objective of this study was to deter- mine whether polymorphisms in the leptin ( LEP ), lep- tin receptor ( LEPR ), paired box 5 ( PAX5 ), aldo-keto reductase ( AKR ), and pro-opiomelanocortin ( POMC ) genes were associated with age, leptin concentration, backfat as an indicator of body condition, or BW at puberty in 3 lines of gilts and to characterize genetic relationships among these traits. The first 2 lines, born in 2001, were formed by crossing maternal White Cross (Yorkshire × Maternal Landrace) gilts to Duroc (n = 210) or (lean) Landrace (n = 207) boars. The remain- ing line (n = 507), born in 2002, was formed by crossing progeny of the Duroc- and Landrace-sired lines. At first estrus, age, BW (BWP), and backfat (BFP) at puberty were recorded and blood was collected for leptin as- says. Nine SNP were detected in candidate genes/re- gions: 1 in LEP , 3 in LEPR , 1 in PAX5 , 2 in AKR , and 2 in POMC . Animals were genotyped for each of the SNP; genotypes were validated using GenoProb. The association model included fixed effects of farrowing group, covariates of SNP genotypic probabilities (from GenoProb), and random additive polygenic effects to account for genetic similarities between animals not explained by SNP. Variance components for polygenic effects and error were estimated using MTDFREML. Leptin concentrations were logarithmically transformed for data analysis. All 4 traits were moderately to highly heritable (0.38 to 0.48). Age and leptin at puberty had a significant ( P < 0.01) genetic correlation at −0.63 ± 0.097, and the genetic correlation between BWP and age at puberty was 0.65 ± 0.083 ( P < 0.01). Significant additive associations (a; P < 0.05) were detected at PAX5 for age at puberty (a = 3.2 d) and for BFP (a = 0.61 mm). One SNP in LEPR was associated with leptin concentration (a = 0.31 log units; P < 0.05). The associations from PAX5 correspond to a QTL peak for age at puberty detected on SSC1. Although not neces- sarily the causative mutation, this result implies that a QTL that can decrease age at puberty without increas- ing BFP and BWP at puberty may exist in this region in commercial pigs.
Image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern