2010-Argente_et_al - Candidate gene analysis for...

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Blasco M. J. Argente, M. Merchán, R. Peiró, M. L. García, M. A. Santacreu, J. M. Folch and A. selected for uterine capacity Candidate gene analysis for reproductive traits in two lines of rabbits divergently doi: 10.2527/jas.2009-2324 originally published online Nov 20, 2009; 2010.88:828-836. J Anim Sci http://jas.fass.org/cgi/content/full/88/3/828 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by on March 5, 2011. jas.fass.org Downloaded from
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ABSTRACT: The objective of this work was to ana- lyze 3 functional candidate genes for reproduction in 2 lines of rabbits divergently selected by uterine capacity. Both lines were selected for 10 generations. The selec- tion was then relaxed until the 17th generation, when it was compounded by 61 and 63 does of the High and Low lines, respectively. We sequenced the SCGB1A1 gene, which encodes the main protein secreted by the rabbit in the uterus and seems to play an important role in implantation. We found 6 SNP in the promoter region cosegregating in 2 haplotypes in both lines with similar frequency. We also analyzed IGF1 mRNA be- cause of its effects on embryo development, but we did not find any polymorphism between individuals of the 2 lines. The third gene analyzed was the TIMP1 , which encodes a protein involved in many biological processes related to reproduction. We determined the sequence of its promoter region and found 1 SNP (g.1423A>G) segregating with different frequencies in both lines (0.60 for allele A in the High line and 0.82 for allele G in the Low line). The association study performed in an F 2 population (n = 598) generated by the cross of the 2 lines of rabbits revealed that the AA genotype had 0.88 embryos more than the GG genotype at 72 h of gestation. The difference increased to 2.23 embryos at implantation, but no difference was found between genotypes at birth. These results suggest that TIMP1 could be a candidate gene for embryo implantation and embryo survival. Key words: embryo survival and development, insulin-like growth factor I gene, litter size, rabbit, secretoglobin family 1A member 1 gene, tissue inhibitor of metalloproteinases 1 gene ©2010 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2010. 88:828–836 doi:10.2527/jas.2009-2324 INTRODUCTION Prenatal losses are a limiting factor in the litter size ( LS ) of rabbits, pigs, and mice. Approximately 30 to 40% of the ova shed do not result in fetuses at term (Blasco et al., 1993), and one-third to one-half of these losses occur before implantation (Ford et al., 2002, in pigs; Holt et al., 2004, in mice; Santacreu et al., 2005, in rabbits). Most of these embryonic losses are charac- terized by an asynchronized development of the embryo with the uterus (see review by Geisert and Schmitt, 2002). Some proteins have an important role in em- bryo development. Secretoglobin family 1A member 1 ( SCGB1A1 , also known as uteroglobin; Riffo et al., 2007), IGF1 (Lin et al., 2003), and tissue inhibitor of metalloproteinases 1 ( TIMP1 ; Hwang et al., 2000) are involved in regulating the embryogenesis, angiogen-
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This note was uploaded on 01/26/2012 for the course ECON 2272 taught by Professor Gay during the Spring '08 term at Birmingham-Southern College.

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2010-Argente_et_al - Candidate gene analysis for...

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