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Unformatted text preview: W. Barendse, R. J. Bunch and B. E. Harrison intramuscular fat percent and marbling score in Australian cattle The effect of variation at the retinoic acid receptor-related orphan receptor C gene on doi: 10.2527/jas.2009-2178 originally published online Oct 9, 2009; 2010.88:47-51. J Anim Sci http://jas.fass.org/cgi/content/full/88/1/47 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by on April 11, 2011. jas.fass.org Downloaded from ABSTRACT: Variation at the retinoic acid receptor- related orphan receptor C ( RORC ) gene was previously associated with marbling score in a large sample of Australian taurine feedlot cattle of Angus and Short- horn breeds. The T allele at the SNP RORC:g.3290T > G increased marbling score in Angus and Shorthorn cattle. We genotyped this SNP in an independent sam- ple of 2,741 Australian cattle of Angus, Brahman, and Hereford breeds, and tested the association of this SNP with marbling score in all animals and with intramus- cular fat (IMF) measurements in 2,104 animals. We found an allele frequency of the G allele of p G = 0.57 in Angus, p G = 0.09 in Hereford, and p G = 0.64 in Brah- man. The regression of marbling score against number of copies of the G allele was significant ( P = 0.033) in the combined sample after accounting for ancestry, breed, and the contemporary group structure of the data. All breeds had the same favorable homozygote; the regression on alleles showed a trend in Angus and Brahman cattle ( P < 0.1), but not in Hereford cattle ( P = 0.912). The regression of IMF against number of copies of the G allele was significant ( P = 0.018) after accounting for ancestry, breed, and contemporary group structure. All breeds had the same favorable ho- mozygote and the regression on alleles was significant ( P = 0.024) in the Angus breed. In all breeds tested in this study, the T allele increased both marbling score and IMF. This polymorphism explained 0.3% of the phenotypic variance for IMF in this sample. Key words: Bos taurus chromosome 3, cattle, deoxyribonucleic acid, intramuscular fat, quantitative trait loci, retinoic acid receptor-related orphan receptor C gene ©2010 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2010. 88:47–51 doi:10.2527/jas.2009-2178 INTRODUCTION Several genes have been associated with QTL for marbling score or intramuscular fat ( IMF ) in cattle, not all of which have been confirmed in all other stud- ies, tested in other studies, or are variants located in plausible candidate genes (Buchanan et al., 2002; Morsci et al., 2006; Cheong et al., 2008). Confirmation of associations in independent studies is an important next step in using the information provided by an as- sociation. The reasons are that 1) the initial association may be a false positive, even for highly significant re- sults; 2) the association will usually be through linkage disequilibrium ( LD ) to the causative mutation, and the...
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This note was uploaded on 01/26/2012 for the course ECON 2272 taught by Professor Gay during the Spring '08 term at Birmingham-Southern College.
- Spring '08
- The Land