2010-Fontanesi_et_al - The insulin-like growth factor 2 (...

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and V. Russo L. Fontanesi, C. Speroni, L. Buttazzoni, E. Scotti, S. DallOlio, L. Nanni Costa, R. Davoli polymorphism carcass traits in pigs: Evidence from the effects of a cathepsin D ( CTSD ) gene not the only Sus scrofa chromosome 2p mutation affecting meat production and The insulin-like growth factor 2 ( IGF2 ) gene intron3-g.3072G>A polymorphism is doi: 10.2527/jas.2009-2560 originally published online April 9, 2010 2010, 88:2235-2245. J ANIM SCI http://jas.fass.org/content/88/7/2235 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by guest on July 16, 2011 jas.fass.org Downloaded from
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ABSTRACT: The objective of this study was to evaluate the effects of mutations in 2 genes [ IGF2 and cathepsin D ( CTSD )] that map on the telomeric end of the p arm of SSC2. In this region, an imprinted QTL affecting muscle mass and fat deposition was reported, and the IGF2 intron3-g.3072G>A substitution was identified as the causative mutation. In the same chro- mosome region, we assigned, by linkage mapping, the CTSD gene, a lysosomal proteinase, for which we pre- viously identified an SNP in the 3 -untranslated region (AM933484, g.70G>A). We have already shown strong effects of this CTSD mutation on several production traits in Italian Large White pigs, suggesting a possible independent role of this marker in fatness and meat deposition in pigs. To evaluate this hypothesis, after having refined the map position of the CTSD gene by radiation hybrid mapping, we analyzed the IGF2 and the CTSD polymorphisms in 270 Italian Large White and 311 Italian Duroc pigs, for which EBV and ran- dom residuals from fixed models were calculated for several traits. Different association analyses were car- ried out to distinguish the effects of the 2 close markers. In the Italian Large White pigs, the results for IGF2 were highly significant for all traits when using either EBV or random residuals (e.g., using EBV: lean cuts, P = 2.2 × 10 −18 ; ADG, P = 2.6 × 10 −16 ; backfat thick- ness, P = 2.2 × 10 −9 ; feed:gain ratio, P = 2.3 × 10 −9 ; ham weight, P = 1.5 × 10 −6 ). No effect was observed for meat quality traits. The IGF2 intron3-g.3072G>A mutation did not show any association in the Italian Duroc pigs, probably because of the small variability at this polymorphic site for this breed. However, a sig- nificant association was evident for the CTSD marker ( P < 0.001) with EBV of all carcass and production traits in Italian Duroc pigs (lean content, ADG, back- fat thickness, feed:gain ratio) after excluding possible confounding effects of the IGF2 mutation. The effects of the CTSD g.70G>A mutation were also confirmed in a subset of Italian Large White animals carrying the homozygous genotype IGF2 intron3-g.3072GG, and by haplotype analysis between the markers of the 2 con- sidered genes in the complete data set. Overall, these results indicate that the IGF2 intron3-g.3072G>A
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This note was uploaded on 01/26/2012 for the course ECON 2272 taught by Professor Gay during the Spring '08 term at Birmingham-Southern College.

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2010-Fontanesi_et_al - The insulin-like growth factor 2 (...

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