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2010-Garcia_et_al - Investigation of the oviductal...

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Santacreu M. L. García, R. Peiró, M. J. Argente, M. Merchán, J. M. Folch, A. Blasco and M. A. survival and development in the rabbit Investigation of the oviductal glycoprotein 1 ( OVGP1 ) gene associated with embryo doi: 10.2527/jas.2009-2042 originally published online January 29, 2010 2010, 88:1597-1602. J ANIM SCI http://jas.fass.org/content/88/5/1597 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by guest on June 13, 2011 jas.fass.org Downloaded from
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ABSTRACT: An association study was performed in rabbits between early embryo survival and development, and the nonconservative SNP 12944C>G located in exon 11 and the triallellic microsatellite [(GT) 15 T(G) 5 , (GT) 14 T(G) 5 , and (GT) 11 T(G) 7 )] located in the promot- er region of the oviductal glycoprotein 1 ( OVGP1 ) gene. We analyzed an F 2 cross of 2 lines of rabbits divergently selected for uterine capacity. A total of 172 and 159 females were slaughtered at 48 and 72 h of gestation, respectively, to determine whether OVGP1 influences ovulation rate, fertilization rate, early embryo survival, and embryonic stage of development. The results of the SNP indicated that all genotypes showed similar early embryo survival and a similar embryonic stage of devel- opment at 48 h of gestation. However, at 72 h of ges- tation, the GG genotype showed greater early embryo survival than the CC genotype (0.56 embryos) and their embryos presented less embryonic development. Analy- sis of the microsatellite was performed to ascertain the presence or absence of the allele (GT) 14 T(G) 5 . At both stages of gestation, the (GT) 14 T(G) 5 /(GT) 14 T(G) 5 gen- otype showed greater early embryo survival (0.94 and 1.54 embryos at 48 and 72 h of gestation, respectively) and less embryonic development than the homozygous genotypes without the allele (GT) 14 T(G) 5 . Key words: association study, embryo survival and development, oviductal glycoprotein 1 ( OVGP1 ), rabbit ©2010 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2010. 88:1597–1602 doi:10.2527/jas.2009-2042 INTRODUCTION Litter size is important for reducing the cost of pro- ducing rabbit meat, and much effort has been put into improving it by direct or indirect selection (Blasco et al., 1994). Ovulation rate ( OR ) and prenatal survival influence litter size in pigs, rabbits, and mice (see Blas- co et al., 1993, for a review). In rabbits, early prenatal survival has been shown to have an important influence on litter size; Torres et al. (1987) reported that em- bryo survival and development before 96 h of gestation was responsible for the difference found in litter size between 2 different rabbit lines. A divergent selection experiment on uterine capacity also showed a difference in early prenatal survival (Mocé et al., 2004; Peiró et al., 2007). At present, a limited number of genes with large or moderate effects on litter size and its components have been associated with SNP in multiparous species (Rothschild et al., 2007). The oviductal glycoprotein 1 ( OVGP1 ) gene is a candidate gene for early prena- tal survival because OVGP1 is mainly expressed in the rabbit oviduct (Merchán et al., 2007). In other species
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