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2010-Kim_et_al - Identification of genetic polymorphisms in...

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E. Kim, H. S. Cheong, J. S. Bae, J. Chun, T. J. Park, K. Lee, Y. Yun and H. D. Shin acid Identification of genetic polymorphisms in bovine mitochondrial deoxyribonucleic doi: 10.2527/jas.2009-2235 originally published online April 23, 2010 2010, 88:2551-2555. J ANIM SCI http://jas.fass.org/content/88/8/2551 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by guest on August 22, 2011 jas.fass.org Downloaded from
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ABSTRACT: In this study, the intent was to iden- tify genetic polymorphisms of mitochondrial (mt) DNA in Korean cattle ( Bos taurus coreana ) and to analyze the genetic relationship between Korean cattle and other breeds. Whole mtDNA genomes (16,338 bp) of 26 animals (16 Korean cattle and 10 Holsteins) were directly sequenced. Multiple alignments, including 26 whole-mtDNA sequences obtained by direct sequencing and 10 mtDNA sequences from a public database (Na- tional Center for Biotechnology Information), revealed 393 mtDNA polymorphisms (382 SNP, 3 heteroplas- mies, and 8 insertion-deletion polymorphisms). Esti- mated gene diversity of mtDNA was 0.00198 among these 36 animals. Phylogenic analysis with mtDNA polymorphisms revealed a distinct genetic difference between Bos taurus (Korean, Japanese Black, Holstein, and Fleckvieh breeds) and Bos indicus (Nellore and Zwergzebu breeds). The genetic information regarding mtDNA polymorphisms identified in this study would be useful for further investigation of mtDNA in other breeds. Key words: Bos taurus coreana , direct sequencing, Korean cattle, mitochondrial deoxyribonucleic acid, phylogenic analysis, polymorphism ©2010 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2010. 88:2551–2555 doi:10.2527/jas.2009-2235 INTRODUCTION More than 800 breeds of cattle are widely distributed throughout the world. The cattle species are thought to have originated from the aurochs, Bos primigenius , which has since been divided into humpless taurine ( Bos taurus ) and humped zebu ( Bos indicus ) through domestication (Epstein, 1971; Epstein and Mason, 1984; MacHugh et al., 1997). Bos taurus includes Ko- rean native, Japanese Black, Holstein, Jersey, Angus, Limousin, Fleckvieh, and other cattle breeds. They are typical breeds of Europe, northeastern Asia, and parts of Africa, and are adapted to cooler climates. Bos in- dicus includes Gir, Guzerat, Kankrej, Indo-Brazilian, Brahman, Nelore, Zwergzebu, and other cattle breeds. They are more common in Africa and South Asia and are adapted to more tropical environments than other domestic cattle. After the completion of human mitochondrial ( mt ) DNA sequencing (Anderson et al., 1981), the complete mt genomes were sequenced for the following additional organisms: common chimpanzees (Horai et al., 1995; Arnason et al., 1996), mice (Bibb et al., 1981), fin whales (Arnason et al., 1991), blue whales, and horses (Arnason and Gullberg, 1993).
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