2010-Peiro_et_al - Expression of progesterone receptor...

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Folch and A. Blasco R. Peiró, A. Herrler, M. A. Santacreu, M. Merchán, M. J. Argente, M. L. García, J. M. the rabbit reproductive tract Expression of progesterone receptor related to the polymorphism in the PGR gene in 2009-11-06T08:41:36-08:00; 2010, 88:421-427.doi: 10.2527/jas.2009-1955 originally published online J ANIM SCI http://jas.fass.org/content/88/2/421 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by guest on May 21, 2011 jas.fass.org Downloaded from
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ABSTRACT: The association of the 2464G > A SNP found in the promoter region of the rabbit progester- one receptor gene with progesterone receptor (PR) ex- pression was evaluated by Western blot analysis. This SNP was associated with 2 lines divergently selected for uterine capacity, the high line selected to increase uterine capacity and the low line selected to decrease uterine capacity. Two progesterone isoforms were ob- tained using a commercial monoclonal antibody: the PR-B isoform described previously in rabbits, and the PR-A isoform, not described previously in rabbits. The GG genotype, the genotype more frequent in the high line, showed less PR-B and PR-A expression than the AA genotype in the oviduct (GG/AA PR-B = 0.81 and GG/AA PR-A = 0.73) and uterus (around 0.70 in both isoforms). The GA genotype showed similar PR-A ex- pression in both tissues and also similar PR-B expres- sion in the oviduct to the GG genotype. Conversely, the GG genotype showed less PR-B expression than the GA genotype in the uterus (GG/GA PR-B = 0.79). Similar expression of both PR isoforms was found in the uterus at d 2 and 3 of gestation; meanwhile, an increase of both isoforms was observed in the oviduct. Similar PR-A expression was observed in the ampulla and isthmus; meanwhile, the PR-B expression in the isthmus was double that in the ampulla. Key words: oviduct, PGR genotype, progesterone receptor expression, rabbit, uterus ©2010 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2010. 88:421–427 doi:10.2527/jas.2009-1955 INTRODUCTION Progesterone is required in mammals for maternal support of conceptus, survival, and development. Most physiological effects of progesterone are applied by its interaction with the specific progesterone receptor ( PR ). In humans, primates, pigs, rodent, and chicken, nuclear PR consist of 2 distinct isoforms, PR-A and PR-B, derived from a single gene independently regu- lated by separate promoters (Kraus et al., 1993; Duffy and Stouffer, 1995). Up to now, only 1 isoform has been described in rabbits (Loosfelt et al., 1984), which has high homology with the PR-B isoform. Different functions have been attributed to each PR isoform. In vitro studies revealed that PR-B functions as a strong activator of transcription of several PR- dependent promoters in which PR-A is inactive. In cultured cells, PR-A can repress the activity of PR-B, as well as other nuclear receptors (Vegeto et al., 1993; Hovland et al., 1998). Studies of knockout mice have also provided insight into different physiological func- tions of each PR isoform for regulating female fertility (Mulac-Jericevic and Conneely, 2004). Selective abla-
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