2010-Souza_et_al - Assessment of DGAT1 and LEP gene...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
R. Ayres, H. Tonhati, S. L. Silva, A. G. Razook and L. G. Albuquerque F. R. P. Souza, M. E. Z. Mercadante, L. F. S. Fonseca, L. M. S. Ferreira, I. C. Regatieri, D. lines selected for growth and their relationship with growth and carcass traits Assessment of DGAT1 and LEP gene polymorphisms in three Nelore ( Bos indicus ) 2009-10-09T07:34:53-07:00; 2010, 88:435-441.doi: 10.2527/jas.2009-2174 originally published online J ANIM SCI http://jas.fass.org/content/88/2/435 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by guest on May 21, 2011 jas.fass.org Downloaded from
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
ABSTRACT: The aim of this study was to analyze LEP and DGAT1 gene polymorphisms in 3 Nelore lines selected for growth and to evaluate their effects on growth and carcass traits. Traits analyzed were birth, weaning, and yearling weight, rump height, LM area, backfat thickness, and rump fat thickness obtained by ultrasound. Two SNP in the LEP gene [ LEP 1620(A/G) and LEP 305(T/C) ] and the K232A mutation in the DGAT1 gene were analyzed. The sample consisted of 357 Nelore heifers from 2 lines selected for yearling weight and a control line, established in 1980, at the Estação Experimental de Zootecnia de Sertãozinho (Sertãozinho, Brazil). Three genotypes were obtained for each marker. Differences in allele frequencies among the 3 lines were only observed for the DGAT1 K232A polymorphism, with the frequency of the A allele being greater in the control line than in the selected lines. The DGAT1 K232A mutation was associated only with rump height, whereas LEP 1620(A/G) was associated with weaning weight and LEP 305(T/C) with birth weight and backfat thickness. However, more studies, with larger data sets, are necessary before these makers can be used for marker-assisted selection. Key words: Bos indicus , molecular marker, productive trait, quantitative trait locus ©2010 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2010. 88:435–441 doi:10.2527/jas.2009-2174 INTRODUCTION In dairy and beef cattle, the K232A mutation in the DGAT1 gene has been associated with greater milk fat content and greater fat content of semitendinosus muscles, respectively (Winter et al., 2002; Thaller et al., 2003; Grisart et al., 2004). It is possible that this mutation is directly responsible for the QTL variation at the centromeric end of BTA14 (Coppieters et al., 1998; Thaller et al., 2003). Several polymorphisms in the LEP gene have been de- scribed in cattle, including LEP 1620(A/G) (GenBank: Y11369) located in intron 2 of the LEP gene (Lien et al., 1997) and LEP 305(T/C) (GenBank: AJ236854) located in exon 2 (Buchanan et al., 2002). For LEP 305(T/C) , the cysteine allele has been associated with greater leptin mRNA levels, increased carcass fat con- tent, and less marbling in beef cattle (Buchanan et al., 2002; Di Stasio et al., 2007). For LEP 1620(A/G) , there have not been any association studies with carcass traits
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/26/2012 for the course ECON 2272 taught by Professor Gay during the Spring '08 term at Birmingham-Southern College.

Page1 / 9

2010-Souza_et_al - Assessment of DGAT1 and LEP gene...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online