2010-Taniguchi_et_al - Fine mapping of quantitative trait...

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Mikawa M. Taniguchi, T. Hayashi, M. Nii, T. Yamaguchi, N. Fujishima-Kanaya, T. Awata and S. 1 gene NUDT7 Analysis of the swine chromosome 6: Sus scrofa Fine mapping of quantitative trait loci for meat color on doi: 10.2527/jas.2009-1814 originally published online Sep 11, 2009; 2010.88:23-31. J Anim Sci http://jas.fass.org/cgi/content/full/88/1/23 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by on April 11, 2011. jas.fass.org Downloaded from
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ABSTRACT: In the livestock industry, meat color has become important because consumer acceptance is subject to the appearance of the product in the market- place. Our previous analyses of a whole genome QTL scan for various meat qualities using 2 F 2 families from Japanese wild boar (known as a red meat) × Large White and from Duroc × Chinese Jinhua suggested that a meat color (heme content) QTL is located on SSC6. The objective of this study was to fine-map this SSC6 meat color QTL and subsequently investigate po- sitional candidate genes for polymorphisms that may cause changes in meat color. Therefore, we conducted interval mapping on SSC6 using an additional 9 gene markers through combined analyses of the 2 F 2 families of Japanese wild boar × Large White (353 progeny) and Duroc × Chinese Jinhua (204 progeny). Compara- tive analysis with humans, mice, and cattle suggest- ed that there were 10 functional genes in the region. Among these genes, we suggested that a novel pig gene encoding a nudix (nucleoside diphosphate linked moi- ety X)-type motif 7 ( NUDT7 , a member of the nudix hydrolases) is a strong candidate for the QTL because the mouse Nudt7 is reported to hydrolyze succinyl- CoA, a substrate of the reaction limiting the rate of heme biosynthesis. We therefore determined the pig NUDT7 gene sequence including the 5 promoter region and explored genetic polymorphisms between Japanese wild boar and Large White. We identified 116 poly- morphisms within the NUDT7 CDS or in the 5 region. None of the AA substitutions were associated with the meat color QTL; however, 3 polymorphisms were found in putative transcription factor recognition sites. We then investigated the differential expression of NUDT7 in Japanese wild boar and Large White by allele-spe- cific quantitative real-time PCR. The expression level of the Large White type allele was greater than that of the Japanese wild-boar-type allele. Consequently, we speculated that the difference in meat color between Japanese wild boar and Large White is caused partly by differential expression of this candidate gene. Up- regulation of NUDT7 expression in muscle may reduce succinyl-CoA content and thus reduce the level of heme biosynthesis. Key words:
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This note was uploaded on 01/26/2012 for the course ECON 2272 taught by Professor Gay during the Spring '08 term at Birmingham-Southern College.

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2010-Taniguchi_et_al - Fine mapping of quantitative trait...

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