{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

2010-Taniguchi_et_al - Fine mapping of quantitative trait...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Mikawa M. Taniguchi, T. Hayashi, M. Nii, T. Yamaguchi, N. Fujishima-Kanaya, T. Awata and S. 1 gene NUDT7 Analysis of the swine chromosome 6: Sus scrofa Fine mapping of quantitative trait loci for meat color on doi: 10.2527/jas.2009-1814 originally published online Sep 11, 2009; 2010.88:23-31. J Anim Sci http://jas.fass.org/cgi/content/full/88/1/23 the World Wide Web at: The online version of this article, along with updated information and services, is located on www.asas.org by on April 11, 2011. jas.fass.org Downloaded from
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
ABSTRACT: In the livestock industry, meat color has become important because consumer acceptance is subject to the appearance of the product in the market- place. Our previous analyses of a whole genome QTL scan for various meat qualities using 2 F 2 families from Japanese wild boar (known as a red meat) × Large White and from Duroc × Chinese Jinhua suggested that a meat color (heme content) QTL is located on SSC6. The objective of this study was to fine-map this SSC6 meat color QTL and subsequently investigate po- sitional candidate genes for polymorphisms that may cause changes in meat color. Therefore, we conducted interval mapping on SSC6 using an additional 9 gene markers through combined analyses of the 2 F 2 families of Japanese wild boar × Large White (353 progeny) and Duroc × Chinese Jinhua (204 progeny). Compara- tive analysis with humans, mice, and cattle suggest- ed that there were 10 functional genes in the region. Among these genes, we suggested that a novel pig gene encoding a nudix (nucleoside diphosphate linked moi- ety X)-type motif 7 ( NUDT7 , a member of the nudix hydrolases) is a strong candidate for the QTL because the mouse Nudt7 is reported to hydrolyze succinyl- CoA, a substrate of the reaction limiting the rate of heme biosynthesis. We therefore determined the pig NUDT7 gene sequence including the 5 promoter region and explored genetic polymorphisms between Japanese wild boar and Large White. We identified 116 poly- morphisms within the NUDT7 CDS or in the 5 region. None of the AA substitutions were associated with the meat color QTL; however, 3 polymorphisms were found in putative transcription factor recognition sites. We then investigated the differential expression of NUDT7 in Japanese wild boar and Large White by allele-spe- cific quantitative real-time PCR. The expression level of the Large White type allele was greater than that of the Japanese wild-boar-type allele. Consequently, we speculated that the difference in meat color between Japanese wild boar and Large White is caused partly by differential expression of this candidate gene. Up- regulation of NUDT7 expression in muscle may reduce succinyl-CoA content and thus reduce the level of heme biosynthesis. Key words: gene expression, meat color, meat quality, quantitative trait loci, swine ©2010 American Society of Animal Science. All rights reserved. J. Anim. Sci. 2010. 88:23–31 doi:10.2527/jas.2009-1814 INTRODUCTION To strengthen consumer acceptance, meat quality improvement has recently become a top priority of the pork industry worldwide. In particular, meat color de-
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}