lecture 10 - 10-1 MCB 354 Protein Function: Myoglobin and...

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10-1 MCB 354 Protein Function: Myoglobin and Hemoglobin Reading in Lehninger : Chapter 5 , pp. 158-165; Ch. 6, pp. 223-227
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The Heme Group Heme = porphyrin Fe2+ atom has 6 coordination bonds: 4 in plane of porphyrin and bonded to N ’s, and 2 perpendicular 10-2
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Two Coordination Positions Perpendicular to Porphyrin Ring One perpendicular bond is to side-chain N on “proximal His”, the other can be to O 2 10-3
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Structure of Myoglobin 10-4 His 93
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Ligand Binding 10-5 [P][L] [PL] Kd = P + L PL θ [L] [L] + Kd = = Binding Sites Occupied Total Binding Sites K d in Units of M Low K d means tighter binding; takes less ligand to reach ½ saturation point
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Proteins Have a Large Range of Kd Values 10-6
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Steric Effects on the Binding of Ligands to Heme 10-7
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Domain Interactions Between Hemoglobin Subunits 10-8
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- all subunits change conformation together , but - binding of ligand increases the probability that all subunits are in the R -form R T R favored R favored R & T ~ equally favored T favored T favored
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lecture 10 - 10-1 MCB 354 Protein Function: Myoglobin and...

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