lecture 12 - 12-1 MCB354 Lecture 12 Km and its Experimental...

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Unformatted text preview: 12-1 MCB354 Lecture 12 Km and its Experimental Determination kcat Reading in Lehninger: Chapter 6, pp. 197-200 12-2 12-3 Michaelis-Menten equation fits experimental observations At [S] << Km, [S] is an insignificant addition to Km, so M-M equation simplifies to: V0 = Vmax [S] Km + [S] At [S] >> Km, Km term in denominator of M-M equation becomes insignificant, and equation simplifies to: ….and V0 has linear dependence on [S]… …..as seen experimentally plateau at high [S] 12-4 12-5 V0 = Vmax [S] Km + [S] 12-6 Determination of Km and Vmax from double reciprocal plot Experimental determination of Km and Vmax: Determine Vo for reaction at a range of [substrate] Plot 1 1 versus Vo [S] From intercepts, calculate Vmax and Km 12-7 12-8 12-9 12-10 Is a function of one or more f irst and/or second order rate processes; and sets a lower limit on the chemical rate constants for the overall rxn . 12-11 AT SATURATION W. [S] 12-12 12-13 12-14 ...
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This note was uploaded on 01/29/2012 for the course MCB 354 taught by Professor Spees during the Spring '09 term at University of Illinois, Urbana Champaign.

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lecture 12 - 12-1 MCB354 Lecture 12 Km and its Experimental...

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