lecture 13 - 13-1MCB354Lecture 13Inhibition of Enzyme...

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Unformatted text preview: 13-1MCB354Lecture 13Inhibition of Enzyme ActivityReading in Lehninger:Chapter 6, pp. 201-205Inhibition of enzyme activityEnzymes can be inhibited by specific small(ish) molecules that interfere withcatalysis and slow or halt a reaction, eg. pharmaceutical agents and toxins1. Irreversible: inhibitorbecomes tightly bound to enzyme-covalently or non-covalentlyEIcomplex dissociates very slowly, if at all2. Reversible:EIcomplex can dissociate rapidly.Different kinds: can be distinguished kinetically13-213-3NOT OTHER SERINES IN THE PROTEINTypes of reversible inhibition1. Competitive: inhibitorbinds to enzyme’sactive site2. Uncompetitive:inhibitorbinds to separate site onenzyme, but only to ES complex3. Noncompetitive (“pure”): inhibitorbinds to separatesite on Eor ES complex with equalaffinity4. Mixed (noncompetitive):inhibitorbinds to separate siteon Eor ES complex with unequalaffinityHave different effects on Kmand Vmax13-4Competitive inhibitors- Often resemble substratestructurally- Compete with...
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This note was uploaded on 01/29/2012 for the course MCB 354 taught by Professor Spees during the Spring '09 term at University of Illinois, Urbana Champaign.

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lecture 13 - 13-1MCB354Lecture 13Inhibition of Enzyme...

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