lecture 22 - MCB 354 Lecture 22 Glycoproteins Glycan...

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MCB 354 Lecture 22 Glycoproteins Glycan Synthesis Carbohydrate Analysis Reading in Lehninger : Chapter 7 , pp. 255-264; Ch. 27 , pp. 1100-1102
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22-1
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Glycoprotein: Protein Oligosaccharide (not GAG) covalently attached to peptide backbone 22-2 Two types of linkage to extracytoplasmic domains of proteins Occur (with variations) in all eukaryotes Rare examples in bacteria and archaea
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22-3 Asparagine-linked (N-) glycoproteins Added to proteins in lumen of rough ER Branched oligosaccharide transferred from lipid carrier (= glycan assembly platform) Oligosacch. trimmed back to varying degrees Different sugars added back in many linkages (in Golgi) Great structural diversity possible w. different sugars , different linkages between them, and branching : ...important in intermolecular recognition: information-rich GlcNAc Man Glc Gal NeuAc α 1,2 β 1,4 α 1 , 6 α 1,2 3 β 1,4 α 1,2 α 1,3 α 1,3 α 1,2 α 1,2 2
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22-4 Stepwise assembly of the oligosaccharide that gets transferred to Asn (DETAILS NOT REQUIRED FOR EXAM) STARTS ON CYTOPLASMIC FACE CARRIER LIPID
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22-5 O-linked glycoproteins Mostly added to proteins in lumen of Golgi Single sugar transferred initially GlcNAc Gal Fucose GalNAc
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( O -glycosidically-linked) force protein into extended conformation covalently attached sugar chain ( N -glycosidically-linked) GPI anchors glycoprotein in membrane) = “Decay accelerating factor” on surface of red blood cells: protects us from our own immune response. Protein
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lecture 22 - MCB 354 Lecture 22 Glycoproteins Glycan...

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